Mouse Fas/CD95 ELISA Kit
Cat no : KE10148
Synonyms
CD95, Fas, APO 1, APT1, TNFR6
Validation Data Gallery
Product Information
KE10148 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Fas/CD95 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Fas/CD95. The assay recognizes mouse Fas/CD95. An antibody specific for mouse Fas/CD95 has been pre-coated onto the microwells. The mouse Fas/CD95 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse Fas/CD95 is added to detect the captured mouse Fas/CD95 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Fas/CD95 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.39-25 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 14102 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 100% | 92%-119% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 7.68 | 0.77 | 10.02 |
| 2 | 8 | 3.16 | 0.11 | 3.46 |
| 3 | 8 | 2.08 | 0.08 | 3.97 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 10.67 | 0.70 | 6.55 |
| 2 | 16 | 3.35 | 0.10 | 3.08 |
| 3 | 16 | 0.90 | 0.07 | 7.78 |
Background Information
Fas ligand (Fas-L) and its receptor Fas (also known as CD95) are members of the tumor necrosis factor family, which interact to play a key role in initiating an apoptotic pathway and accounting for the molecular mechanism of activation-induced T-cell death. They may be linked to breast cancer progression. Fas can be a therapeutic target in rheumatoid arthritis as well. Serum-soluble Fas and soluble Fas-L levels were significantly higher in some rheumatic disease patients. It also has been reported that increased soluble CD95 (sFas/CD95) serum level correlates with poor prognosis in melanoma patients.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD95, Fas, APO 1, APT1, TNFR6 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.