Mouse Foxp3 ELISA Kit
Cat no : KE10210
Synonyms
forkhead box P3, Forkhead box protein P3 41 kDa form, Forkhead box protein P3, C-terminally processed, JM2, scurfin
Validation Data Gallery
Product Information
KE10210 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Foxp3 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Foxp3. The assay recognizes mouse Foxp3. An antibody specific for mouse Foxp3 has been pre-coated onto the microwells. The mouse Foxp3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse Foxp3 is added to detect the captured mouse Foxp3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Foxp3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 10.2 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20371 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 111% | 102%-122% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,671.8 | 86.4 | 3.2 |
| 2 | 8 | 573.8 | 18.1 | 3.1 |
| 3 | 8 | 124.1 | 5.4 | 4.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,611.4 | 125.2 | 4.8 |
| 2 | 16 | 563.1 | 19.7 | 3.5 |
| 3 | 16 | 122.5 | 7.2 | 5.9 |
Background Information
Foxp3 (Forkhead box P3) is a transcription factor that belongs to the Forkhead box family. It is a key transcription factor for regulatory T cells (Tregs) and is crucial for maintaining immune system homeostasis. Deficiency in Foxp3 can lead to immune dysregulation, polyendocrinopathy, enteropathy, and X-linked syndrome (IPEX). In mice, the expression of Foxp3 is primarily localized in the nuclei of regulatory T cells. This protein maintains immune system homeostasis by regulating the stability and suppressive function of Tregs, as well as directly regulating the proliferation and function of conventional T cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | forkhead box P3, Forkhead box protein P3 41 kDa form, Forkhead box protein P3, C-terminally processed, JM2, scurfin |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.