Mouse GSDMD ELISA Kit
Cat no : KE10164
Synonyms
AW558049, DF5L, Dfna5l, gasdermin D, Gsdmdc1
Validation Data Gallery
Product Information
KE10164 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse GSDMD ELISA kit is to be used to detect and quantify protein levels of endogenous mouse GSDMD. The assay recognizes mouse GSDMD. An antibody specific for mouse GSDMD has been pre-coated onto the microwells. The mouse GSDMD protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse GSDMD is added to detect the captured mouse GSDMD protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse GSDMD ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 69146 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 90% | 81%-103% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.78 | 0.14 | 2.84 |
| 2 | 8 | 1.25 | 0.04 | 3.21 |
| 3 | 8 | 0.60 | 0.03 | 4.23 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.08 | 0.35 | 6.96 |
| 2 | 16 | 1.35 | 0.11 | 8.18 |
| 3 | 16 | 0.63 | 0.04 | 6.47 |
Background Information
Gasdermin D (GSDMD) is a member of the gasdermin family and a key pore-forming protein that plays a crucial role in regulating inflammatory responses and various forms of cell death, particularly pyroptosis. GSDMD is activated by inflammatory caspases, such as caspase-1, -4, -5, and -11, which cleave it into an N-terminal fragment (GSDMD-N) and a C-terminal fragment (GSDMD-C). GSDMD has both pro-inflammatory and protective roles depending on the context. In some infectious diseases and inflammatory conditions, GSDMD helps in pathogen clearance and immune defense. In tumor diseases, GSDMD exhibits dual regulatory roles: it can suppress tumor growth but may also promote tumor cell migration and metastasis under certain conditions. Targeting GSDMD has emerged as a promising therapeutic strategy for various inflammatory and non-tumor diseases
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AW558049, DF5L, Dfna5l, gasdermin D, Gsdmdc1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.