Mouse HER2/ErbB2 ELISA Kit
Cat no : KE10168
Synonyms
CD340, EC:2.7.10.1, Erbb2, Her2, Her2 / ErbB2
Validation Data Gallery
Product Information
KE10168 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse HER2/ErbB2 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse HER2/ErbB2. The assay recognizes mouse HER2/ErbB2. An antibody specific for mouse HER2/ErbB2 has been pre-coated onto the microwells. The mouse HER2/ErbB2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse HER2/ErbB2 is added to detect the captured mouse HER2/ErbB2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse HER2/ErbB2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 13866 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 105% | 96%-125% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.44 | 0.20 | 3.64 |
| 2 | 8 | 1.41 | 0.02 | 1.25 |
| 3 | 8 | 0.75 | 0.03 | 3.67 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.59 | 0.22 | 3.88 |
| 2 | 16 | 1.33 | 0.10 | 7.17 |
| 3 | 16 | 0.73 | 0.03 | 3.88 |
Background Information
HER2/ErbB2 is a critical receptor tyrosine kinase protein belonging to the epidermal growth factor receptor (EGFR) family, which also includes HER1 (EGFR), HER3, and HER4. Unlike other members of the EGFR family, HER2/ErbB2 does not have a known ligand and is often referred to as an "orphan receptor." However, it can form homodimers or heterodimers with other EGFR family members, such as HER1, HER3, and HER4, significantly enhancing its signaling potential. This receptor is involved in the regulation of multiple downstream signaling pathways, including the PI3K-Akt and MAPK pathways, which promote cell survival, proliferation, and migration.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD340, EC:2.7.10.1, Erbb2, Her2, Her2 / ErbB2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.