Mouse IGFBP3 ELISA Kit
Cat no : KE10078
Synonyms
IBP-3, IGF-binding protein 3, IGFBP-3, IGgfbp3, Insulin-like growth factor-binding protein 3
Validation Data Gallery
Product Information
KE10078 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IGFBP3 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IGFBP3. The assay recognizes mouse IGFBP3. An antibody specific for mouse IGFBP3 has been pre-coated onto the microwells. The mouse IGFBP3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IGFBP3 is added to detect the captured mouse IGFBP3 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IGFBP3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 6.9 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16009 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 95% | 83%-114% |
| Cell culture supernatants | 79% | 70%-93% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,098.5 | 47.3 | 2.3 |
| 2 | 20 | 505.1 | 12.1 | 2.4 |
| 3 | 20 | 126.5 | 4.3 | 3.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,006.4 | 94.2 | 4.7 |
| 2 | 24 | 496.9 | 13.9 | 2.8 |
| 3 | 24 | 120.2 | 7.4 | 6.1 |
Background Information
Insulin-like growth factor binding protein 3, IGFBP3, is an N-linked glycosylated, phosphorylated, secretory protein with known antiproliferative and pro-apoptotic functions. IGFBP3 belongs to a family of high affinity insulin-like growth factor (IGF) binding proteins, which function to sequester extracellular IGF-1, preventing IGF-1 activation of the insulin-like growth factor receptor, IGF-1R. IGFBP3 is highly expressed in the endometrium and at the maternal-fetal interface in multiple species, including humans. In a mouse embryo coculture system,IGFBP3 was found to be secretedby endometrial cells that promote embryonic developmentby enhancing the effect of IGF.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IBP-3, IGF-binding protein 3, IGFBP-3, IGgfbp3, Insulin-like growth factor-binding protein 3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.