Mouse IL-1R1 ELISA Kit
Cat no : KE10169
Synonyms
CD121 antigen-like family member A, CD121a, EC:3.2.2.6, Il1r1, IL-1R-1
Validation Data Gallery
Product Information
KE10169 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-1R1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-1R1. The assay recognizes mouse IL-1R1. An antibody specific for mouse IL-1R1 has been pre-coated onto the microwells. The mouse IL-1R1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse IL-1R1 is added to detect the captured mouse IL-1R1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-1R1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 29.1 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16177 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 100% | 93%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,406.9 | 43.3 | 1.8 |
| 2 | 8 | 588.4 | 27.4 | 4.7 |
| 3 | 8 | 326.5 | 14.9 | 4.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,410.7 | 41.5 | 1.7 |
| 2 | 16 | 579.4 | 26.5 | 4.6 |
| 3 | 16 | 323.2 | 12.9 | 4.0 |
Background Information
The IL-1R family plays roles in the regulation of innate inflammation, host defense, and acquired immunity. Interleukin-1 receptor type 1 (IL-1R1) is expressed by all cells responsive to IL-1, the predominant type of IL-1R on T cells, fibroblasts, epithelial and endothelial cells. IL-1R1 activation promotes Ly6G expressing neutrophil accumulation in the injured kidney and generation of tumor necrosis factor (TNF) by intrarenal myeloid cells. The soluble form of IL-1R1 acts as a decoy inhibitor by capturing IL-1 cytokines in solution.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD121 antigen-like family member A, CD121a, EC:3.2.2.6, Il1r1, IL-1R-1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.