Mouse IL-1R2 ELISA Kit
Cat no : KE10213
Synonyms
CD121 antigen-like family member B, CD121b, IL-1 type II receptor, Il1r2, IL-1R-2
Validation Data Gallery
Product Information
KE10213 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-1R2 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-1R2. The assay recognizes mouse IL-1R2. An antibody specific for mouse IL-1R2 has been pre-coated onto the microwells. The mouse IL-1R2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse IL-1R2 is added to detect the captured mouse IL-1R2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-1R2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 13.5 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16178 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 106% | 102%-112% |
| Tissue lysate | 107% | 103%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,108.0 | 49.0 | 4.4 |
| 2 | 8 | 259.7 | 12.7 | 4.9 |
| 3 | 8 | 140.7 | 5.4 | 3.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,134.5 | 52.8 | 4.7 |
| 2 | 16 | 267.3 | 15.2 | 5.7 |
| 3 | 16 | 145.8 | 7.9 | 5.4 |
Background Information
Interleukin-1 receptor type 2 (IL-1R2, also known as CD121b) is a receptor for the pro-inflammatory cytokines IL-1α and IL-1β. It plays a crucial role in the negative regulation of the IL-1 signaling pathway, which is involved in inflammation and immune responses. IL-1R2 also regulates IL-1 maturation and cell activation, promotes cell survival, and participates in IL-1-dependent internalization. Soluble IL-1R2 (sIL-1R2) acts as a decoy receptor by binding to IL-1α and IL-1β, preventing their interaction with IL-1R1 and IL-1RAP. This inhibits the activation of the IL-1 signaling pathway, thereby reducing inflammation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD121 antigen-like family member B, CD121b, IL-1 type II receptor, Il1r2, IL-1R-2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.