Mouse IL-2 ELISA Kit
Cat no : KE10126
Synonyms
Il2,Il-2,Il 2,interleukin 2,Interleukin-2
Validation Data Gallery
Product Information
KE10126 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-2 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-2. The assay recognizes mouse IL-2. An antibody specific for mouse IL-2 has been pre-coated onto the microwells. The mouse IL-2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse IL-2 is added to detect the captured mouse IL-2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 2.3 pg/mL |
| Range | 12.5-800 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16183 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 92% | 70%-117% |
| Cell culture supernatant | 101% | 77%-115% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 393.6 | 13.4 | 3.4 |
| 2 | 20 | 95.3 | 5.3 | 5.5 |
| 3 | 20 | 22.0 | 1.7 | 7.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 392.4 | 12.4 | 3.2 |
| 2 | 24 | 95.3 | 3.0 | 3.1 |
| 3 | 24 | 22.8 | 1.3 | 5.6 |
Background Information
Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function and development, maintenance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signal is mediated through heterodimerization of the IL2Rβ and Il-2Rγ receptor subunits.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il2,Il-2,Il 2,interleukin 2,Interleukin-2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
ACS Nano 2240 nm NIR-IV Photodynamic Therapy Can Reverse Ineffective Anti-OX40 Cancer Immunotherapy to Become Effective. | ||
Adv Sci (Weinh) A Spatially Distributed Microneedle System for Bioorthogonal T Cell-Guided Cancer Therapy | ||
Asian J Pharm Sci Mesoporous polydopamine nanoplatform enhances IL-2 immunotherapy for hepatocellular carcinoma via mild photothermal therapy and lactate regulation. | ||
J Colloid Interface Sci Engineered cell nanovesicle antagonists for androgen deprivation therapy of melanoma | ||
Int Immunopharmacol MFAP4 activates PI3K/AKT-NF-κB signaling to drive tumor progression and immune evasion in bladder cancer. | ||
Int Immunopharmacol Dose-dependent efficacy of CX1003 in NSCLC immunotherapy: High dose for T cell response, low dose for apoptosis |