Mouse IL-1 alpha ELISA Kit
Cat no : KE10024
Synonyms
Il1a, IL1-alpha, Il 1a, IL1 alpha, IL-1,IL1
Validation Data Gallery
Product Information
KE10024 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-1 alpha ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1 alpha. The assay recognizes mouse IL-1 alpha. An antibody specific for IL-1 alpha has been pre-coated onto the microwells. The IL-1 alpha protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-1 alpha is added to detect the captured IL-1 alpha protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-1 alpha ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 2.7 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16175 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 87% | 77%-103% |
| Cell culture supernatants | 101% | 94%-118% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 33.9 | 2.1 | 6.1 |
| 2 | 20 | 95.0 | 3.5 | 3.7 |
| 3 | 20 | 388.7 | 15.0 | 3.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 37.0 | 3.6 | 9.6 |
| 2 | 24 | 117.6 | 7.3 | 6.3 |
| 3 | 24 | 504.9 | 45.8 | 9.1 |
Background Information
Interleukin-1α (Il-1α) is a protein that plays a crucial role in the regulation of a number of key cellular processes. Il-1α is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that Il-1α also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. Il-1α functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. Il-1α was found to affect the cell cycle of osteosarcoma cell to reduce cell growth.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il1a, IL1-alpha, Il 1a, IL1 alpha, IL-1,IL1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Cell Mol Gastroenterol Hepatol ANKRD22 Drives Rapid Proliferation of Lgr5+ Cells and Acts as a Promising Therapeutic Target in Gastric Mucosal Injury. | ||
Cancer Immunol Immunother ANKRD22 participates in the proinflammatory activities of macrophages in the colon cancer tumor microenvironment | ||
Front Immunol Inhibitory Effects of the Lactobacillus rhamnosus GG Effector Protein HM0539 on Inflammatory Response Through the TLR4/MyD88/NF-кB Axis. | ||
Sci Rep Phasic firing of dopaminergic neurons in the ventral tegmental area triggers peripheral immune responses. | ||
BMC Complement Med Ther Photoprotective effects of Sargassum thunbergii on ultraviolet B-induced mouse L929 fibroblasts and zebrafish. | ||
Cells Co-Stimulation of AGEs and LPS Induces Inflammatory Mediators through PLCγ1/JNK/NF-κB Pathway in MC3T3-E1 Cells |