Mouse IL-12 p70 ELISA Kit
Cat no : KE10014
Synonyms
Il 12a, IL 12p35, IL-12, IL12 p70, IL-12 p70, Il12a, IL12p70, IL12-p70, interleukin 12a, Interleukin-12, Ll12a, p35
Validation Data Gallery
Product Information
K10014 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-12 p70 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-12 p70. The assay recognizes mouse IL-12 p70. An antibody specific for mouse IL-12 p70 has been pre-coated onto the microwells. The mouse IL-12 p70 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-12 p70 is added to detect the captured mouse IL-12 p70 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-12 p70 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 2.2 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16159 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 101% | 84%-115% |
| Cell culture supernatants | 87% | 78%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 821.4 | 55.5 | 6.8 |
| 2 | 20 | 191.8 | 6.4 | 3.4 |
| 3 | 20 | 19.7 | 1.1 | 5.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 776.8 | 52.9 | 6.8 |
| 2 | 24 | 191.5 | 12.0 | 6.2 |
| 3 | 24 | 18.7 | 1.1 | 6.0 |
Background Information
Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 (referred to as 'p70') is a 70 kDa heterodimer composed of a 35 kDa subunit (IL-12a p35) and a 40 kDa subunit (IL-12b p40). IL-12 is involved in the differentiation of native T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. IL-12 has been shown to play a critical role in the pathogenesis of a variety of immune-related diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il 12a, IL 12p35, IL-12, IL12 p70, IL-12 p70, Il12a, IL12p70, IL12-p70, interleukin 12a, Interleukin-12, Ll12a, p35 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Proc Natl Acad Sci U S A miR-182 targeting reprograms tumor-associated macrophages and limits breast cancer progression. | ||
Oncogene Immunosuppressive effects of hypoxia-induced glioma exosomes through myeloid-derived suppressor cells via the miR-10a/Rora and miR-21/Pten Pathways. | ||
Cell Mol Gastroenterol Hepatol ANKRD22 Drives Rapid Proliferation of Lgr5+ Cells and Acts as a Promising Therapeutic Target in Gastric Mucosal Injury. | ||
Int J Mol Sci Human MIKO-1, a Hybrid Protein That Regulates Macrophage Function, Suppresses Lung Fibrosis in a Mouse Model of Bleomycin-Induced Interstitial Lung Disease | ||
Front Pharmacol A Polysaccharide From the Whole Plant of Plantago asiatica L. Enhances the Antitumor Activity of Dendritic Cell-Based Immunotherapy Against Breast Cancer | ||
Life Sci The impact of ExHp-CD (outer membrane vesicles) released from Helicobacter pylori SS1 on macrophage RAW 264.7 cells and their immunogenic potential. |