Mouse IL-17A ELISA Kit
Cat no : KE10020
Synonyms
Ctla 8, Ctla8, CTLA-8, Il17, IL-17, Il17a, IL-17A, interleukin 17A, Interleukin-17A
Validation Data Gallery
Product Information
KE10020 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL-17A ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-17A. The assay recognizes mouse IL-17A. An antibody specific for mouse IL-17A has been pre-coated onto the microwells. The mouse IL-17A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-17A is added to detect the captured mouse IL-17A protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-17A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Tissue lysates |
| Assay type | Sandwich |
| Sensitivity | 2.6 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16171 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 93% | 74%-112% |
| Cell culture supernatants | 93% | 87%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 9.9 | 0.8 | 8.0 |
| 2 | 20 | 104.5 | 4.5 | 4.3 |
| 3 | 20 | 386.5 | 26.1 | 6.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 10.2 | 1.0 | 9.6 |
| 2 | 24 | 93.7 | 8.6 | 9.2 |
| 3 | 24 | 428.2 | 42.1 | 9.8 |
Background Information
Il17a, also named as Il-17, is a proinflammatory cytokine. Il-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The Il-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. Il-17 and IL-22, are highly increased.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ctla 8, Ctla8, CTLA-8, Il17, IL-17, Il17a, IL-17A, interleukin 17A, Interleukin-17A |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Redox Biol Aryl hydrocarbon receptor in club cells drives Th17-mediated lung injury following inhalation exposure to environmentally persistent free radicals. | ||
J Nanobiotechnology Green Phellodendri Chinensis Cortex-based carbon dots for ameliorating imiquimod-induced psoriasis-like inflammation in mice. | ||
J Control Release Dual-action ROS-responsive nanoparticles for synergistic suppression of pyroptosis and cfDNA in inflammatory bowel diseases. | ||
Phytomedicine Scutellaria baicalensis water decoction ameliorates lower respiratory tract infection by modulating respiratory microbiota | ||
J Hazard Mater Ozone-induced renal homeostasis disruption in diabetic mice: The role of Th17/Treg imbalance and drug intervention. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Rashmi (Verified Customer) (11-24-2025) | Used this kit to assay for IL17A levels in mouse BALF samples (1:2 diluted). The assay worked well with these samples.
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