Mouse IL-5 ELISA Kit
Cat no : KE10018
Synonyms
Il5, IL-5, Il 5, interleukin 5, interleukin-5
Validation Data Gallery
Product Information
KE10018 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-5 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-5. The assay recognizes mouse IL-5. An antibody specific for IL-5 has been pre-coated onto the microwells. The IL-5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-5 is added to detect the captured IL-5 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-5 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.0 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16191 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 90% | 76%-116% |
| Cell culture supernatants | 94% | 82%-108% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 10.3 | 0.4 | 3.9 |
| 2 | 20 | 108.0 | 2.0 | 1.9 |
| 3 | 20 | 556.4 | 21.6 | 4.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 10.4 | 0.5 | 4.9 |
| 2 | 24 | 111.4 | 1.8 | 1.6 |
| 3 | 24 | 503.0 | 17.4 | 3.5 |
Background Information
Interleukin-5, or IL-5, was originally discovered as a soluble T cell-derived factor, called T cell-replacing factor (TRF), that induced T cell-depleted activated B cells to secrete immunoglobulin. IL-5 is a key hematopoietic cytokine in eosinophil differentiation, maturation, recruitment and activation at sites of allergic inflammation. IL-5 also plays a role in the development, metabolism, and function of basophils. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of at least two chains: an α chain that binds IL-5 with low affinity and a βchain that does not bind IL-5, but together with the IL-5α chain, constitutes the high affinity IL-5 receptor. The β chain is common to the IL-3, IL-5 and Gm-csf receptors and has been shown to signal through the JAK/Stat pathway.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il5, IL-5, Il 5, interleukin 5, interleukin-5 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Int Immunopharmacol Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma | ||
Immunology BCG Vaccination Reprograms the Function of M-MDSCs and Aggravates Necrotizing Enterocolitis in Neonates | ||
Immunol Invest miR-142-3p Regulates Airway Inflammation Through PTEN/AKT in Children and Mice with Asthma | ||
Basic Clin Pharmacol Toxicol SIRT1/IGFBPrP1/TGF β1 axis involved in cucurbitacin B ameliorating concanavalin A-induced mice liver fibrosis. | ||
Chem Biodivers Exploring the Active Substances and Mechanism of Physalis Calyx seu Fructus Against Inflammation-related Respiratory Diseases via Integrating Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry, Network Pharmacological Analysis, and Experimental Verification. | ||
J Food Biochem Lycoperoside H, a steroidal alkaloid saponin in tomato seeds, ameliorates atopic dermatitis-like symptoms in IL-33 transgenic mice. |