Speedy™ Mouse IgE One-Step ELISA Kit
Cat no : SE60120
Synonyms
Ig epsilon chain C region, IGHE
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse IgE One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Tissue lysates |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 380792 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 101% | 98%-103% |
| Tissue lysate | 96% | 92%-98% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 8.73 | 0.14 | 1.60 |
| 2 | 8 | 2.51 | 0.04 | 1.59 |
| 3 | 8 | 1.37 | 0.04 | 2.92 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 8.63 | 0.17 | 1.97 |
| 2 | 16 | 2.53 | 0.05 | 1.98 |
| 3 | 16 | 1.36 | 0.04 | 2.94 |
Background Information
Immunoglobulin E (IgE) is a specialized type of antibody produced by the immune system that plays a pivotal role in defending against parasitic infections (such as helminths) and mediating allergic reactions. Although it is the least abundant antibody isotype in human serum, IgE triggers powerful immune responses by binding to high-affinity receptors on mast cells and basophils. Upon subsequent exposure to a specific allergen, this binding prompts the rapid release of inflammatory chemical mediators like histamine, leading to the classic symptoms of allergic asthma, hay fever, and anaphylaxis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ig epsilon chain C region, IGHE |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.