Speedy™ Mouse IgM One-Step ELISA Kit
Cat no : SE60117
Synonyms
Igh-6, Ighm, Immunoglobulin heavy constant mu, immunoglobulin M, Mouse IgM
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse IgM One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16019 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 99% | 89%-105% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.21 | 0.24 | 4.61 |
| 2 | 8 | 1.30 | 0.03 | 2.31 |
| 3 | 8 | 0.65 | 0.05 | 7.69 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.55 | 0.62 | 11.17 |
| 2 | 16 | 1.35 | 0.08 | 5.93 |
| 3 | 16 | 0.66 | 0.05 | 7.58 |
Background Information
IgM is one of several isotypes of antibody (immunoglobulin) produced by the immune system and is the first antibody to be generated in response to an initial exposure to an antigen. In clinical medicine, the detection of IgM antibodies against a specific pathogen (such as a virus or bacteria) usually indicates a recent infection or the acute phase of a disease. As the immune response progresses, IgM levels typically decline and are replaced by longer-lasting IgG antibodies.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Igh-6, Ighm, Immunoglobulin heavy constant mu, immunoglobulin M, Mouse IgM |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.