Mouse Insulin ELISA Kit
Cat no : KE10089
Synonyms
Ins 1, Ins1, Ins2 rs1, insulin I
Validation Data Gallery
Product Information
KE10089 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Insulin ELISA kit is to be used to detect and quantify protein levels of endogenous insulin. The assay recognizes mouse insulin. An antibody specific for insulin has been pre-coated onto the microwells. The insulin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for insulin is added to detect the captured insulin protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Insulin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum |
| Assay type | Sandwich |
| Sensitivity | 1.3 pg/mL |
| Range | 6.25-400 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16333 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 93% | 77%-106% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 202.7 | 8.0 | 4.0 |
| 2 | 20 | 48.8 | 2.0 | 4.1 |
| 3 | 20 | 11.8 | 0.9 | 7.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 197.2 | 13.2 | 6.7 |
| 2 | 24 | 48.7 | 2.1 | 4.4 |
| 3 | 24 | 12.6 | 0.9 | 7.3 |
Background Information
Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in insulin results in type 1 diabetes mellitus.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ins 1, Ins1, Ins2 rs1, insulin I |
Publications
| Species | Sample Type | Title |
|---|---|---|
Phytomedicine Combinational application of the natural products 1-deoxynojirimycin and morin ameliorates insulin resistance and lipid accumulation in prediabetic mice | ||
PeerJ Profile of the bile acid FXR-FGF15 pathway in the glucolipid metabolism disorder of diabetic mice suffering from chronic stress | ||
World J Gastroenterol Ferroptosis inhibition attenuates inflammatory response in mice with acute hypertriglyceridemic pancreatitis |