Mouse Leptin ELISA Kit

Sensitivity

0.4 pg/mL

Range

62.5 - 4000 pg/mL

Reactivity

Mouse

Cat no : KE10048

Synonyms

Lep, leptin, ob, obese



Product Information

KE10048 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse Leptin ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Leptin. The assay recognizes mouse Leptin. An antibody specific for mouse Leptin has been pre-coated onto the microwells. The mouse Leptin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse Leptin is added to detect the captured mouse Leptin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse Leptin ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 0.4 pg/mL
Range 62.5 - 4000 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 16846

Recovery

Sample TypeAverageRange
Mouse serum103%85%-128%
Cell culture supernatants102%90%-114%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1202,076.178.23.8
220535.515.32.9
320160.74.83.0

InterAssay

Samplenmean ( pg/mL)SDCV%
1241,377.9142.210.3
224354.733.79.5
32482.18.710.6

Background Information

Leptin is a 16 kDa adipocyte-derived hormone, and it has attracted interest in the field of obesity research due to its role in the regulation of energy balance. Leptin, secreted from adipose tissues in proportion to the fat store, acts in the hypothalamus to regulate feeding behavior. leptin has also been shown to play an essential role in tumorigenesis, tumor progression, and metastasis, due to its oncogenic, mitogenic, pro-inflammatory, and pro-angiogenic actions. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Lep, leptin, ob, obese

Publications

SpeciesSample TypeTitle
mouseserum

J Cachexia Sarcopenia Muscle

Pim1 knockout alleviates sarcopenia in aging mice via reducing adipogenic differentiation of PDGFRα+ mesenchymal progenitors.

Authors - Guo-Kai Shang
mouseserum

J Nutr Biochem

Triacylglycerol rich in docosahexaenoic acid regulated appetite via the mediation of leptin and intestinal epithelial functions in high-fat, high-sugar diet-fed mice.

Authors - Wanxiu Cao
mouseserum

J Immunol Res

Obesity Potentiates Esophageal Squamous Cell Carcinoma Growth and Invasion by AMPK-YAP Pathway.

Authors - Jia-Huang Liu
mouseserum

Front Endocrinol (Lausanne)

Corticosterone induces obesity partly via promoting intestinal cell proliferation and survival

Authors - Guanhao Wang