Mouse MCP-1/CCL2 ELISA Kit
Cat no : KE10006
Synonyms
Ccl2, MCP1, MCP-1, C-C motif chemokine 2, CCL 2
Validation Data Gallery
Product Information
KE10006 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MCP-1 ELISA kit is to be used to detect and quantify protein levels of endogenous MCP-1. The assay recognizes mouse MCP-1. An antibody specific for MCP-1 has been pre-coated onto the microwells. The MCP-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for MCP-1 is added to detect the captured MCP-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse MCP-1/CCL2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.3 pg/mL |
| Range | 3.13-200 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20296 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 91% | 76%-127% |
| Cell culture supernatants | 102% | 85%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 196.2 | 17.4 | 8.8 |
| 2 | 20 | 52.3 | 2.9 | 5.5 |
| 3 | 20 | 11.2 | 0.7 | 6.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 110.1 | 9.2 | 8.4 |
| 2 | 24 | 33.1 | 2.6 | 8.0 |
| 3 | 24 | 8.5 | 0.7 | 8.4 |
Background Information
Monocyte chemotactic protein 1 (Mcp1; also known as Ccl2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ccl2, MCP1, MCP-1, C-C motif chemokine 2, CCL 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Adv Sci (Weinh) H3K36me3-Guided m6A Modification of Oncogenic L1CAM-AS1 Drives Macrophage Polarization and Immunotherapy Resistance in Hepatocellular Carcinoma | ||
Bone Res Osteopontin deficiency promotes cartilaginous endplate degeneration by enhancing the NF-κB signaling to recruit macrophages and activate the NLRP3 inflammasome | ||
Nat Commun Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH) | ||
ACS Appl Mater Interfaces Prussian Blue Nanozymes Prevent Anthracycline-Induced Liver Injury by Attenuating Oxidative Stress and Regulating Inflammation. | ||
Adv Healthc Mater Enhanced Antibacterial And Wound Healing Using A Metal-Organic Cluster Inspired by Artificial Photosynthesis | ||
Int J Biol Sci Olfactomedin-4 deletion exacerbates DSS-induced colitis through a matrix metalloproteinase-9-dependent mechanism |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Celine (Verified Customer) (09-10-2025) | This ELISA kit was used to quantify MCP-1 levels in frozen murine plasma samples. The kit was easy to use and the instructions were clear. The kit came with 2 sets of standards so we were able to run a trial run on a few samples before going ahead with our experimental samples.
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