Mouse MCP-1/CCL2 ELISA Kit
Cat no : KE10102
Synonyms
Ccl2, MCP1, MCP-1, C-C motif chemokine 2, CCL 2
Validation Data Gallery
Product Information
KE10102 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse MCP-1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse MCP-1. The assay recognizes mouse MCP-1. An antibody specific for mouse MCP-1 has been pre-coated onto the microwells. The mouse MCP-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse MCP-1 is added to detect the captured mouse MCP-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse MCP-1/CCL2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 1.3 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20296 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 103% | 87%-118% |
| Cell culture supernatant | 95% | 84%-105% |
| Tissue lysate | 105% | 94%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 221.1 | 5.8 | 2.6 |
| 2 | 20 | 54.1 | 1.6 | 2.9 |
| 3 | 20 | 12.0 | 0.8 | 6.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 227.7 | 11.6 | 5.1 |
| 2 | 24 | 54.4 | 2.3 | 4.3 |
| 3 | 24 | 12.8 | 0.7 | 5.4 |
Background Information
Monocyte chemotactic protein 1 (MCP1; also known as CCL2), is a chemokine that can be expressed in monocytes, macrophages, and endothelial cells, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. Research has shown that the expression of MCP1 increases in the serum of patients with acute myocarditis. MCP1 is up-regulated in many types of CNS injury, including ischemia, hemorrhage, trauma, infection, hypoxia, and peripheral nerve axotomy. MCP1 has also been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ccl2, MCP1, MCP-1, C-C motif chemokine 2, CCL 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.