Mouse MCP-1/CCL2 ELISA Kit
Cat no : KE10123
Synonyms
Ccl2, MCP1, MCP-1, C-C motif chemokine 2, CCL 2
Validation Data Gallery
Product Information
KE10123 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse MCP-1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse MCP-1. The assay recognizes mouse MCP-1. An antibody specific for mouse MCP-1 has been pre-coated onto the microwells. The mouse MCP-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse MCP-1 is added to detect the captured mouse MCP-1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse MCP-1/CCL2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 1.2 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20296 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 110% | 79%-129% |
| Cell culture supernatant | 95% | 85%-117% |
| Tissue lysate | 100% | 92%-115% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 509.8 | 10.8 | 2.1 |
| 2 | 20 | 119.1 | 3.2 | 2.7 |
| 3 | 20 | 29.1 | 1.5 | 5.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 508.0 | 33.1 | 6.5 |
| 2 | 24 | 122.5 | 6.6 | 5.4 |
| 3 | 24 | 29.8 | 1.9 | 6.2 |
Background Information
Monocyte chemotactic protein 1 (MCP-1; also known as CCL2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ccl2, MCP1, MCP-1, C-C motif chemokine 2, CCL 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Mol Psychiatry Aspirin alleviates long-term high-fat diet-induced depressive-like behavior in male mice via suppressing arachidonic acid-mediated microglial activation and neuroinflammation. | ||
Aging Cell A Non-Channel Function of CFTR: Attenuating Mitochondrial Oxidative Stress and Cardiomyocyte Senescence via Stabilization by USP45. | ||
Biochem Pharmacol Targeting PIM1 ameliorates sepsis-associated acute kidney injury through inhibiting ferroptosis. | ||
Life Sci Inhibiting SIRT2 attenuates sepsis-associated acute kidney injury via NEU1 acetylation-mediated CD44/PI3K/AKT pathway activation. | ||
Eur J Pharmacol Glycin-proline-hydroxyproline modified N-terminal 20 peptide from CEMP1 blocks calcium ion influx to alleviate inflammatory response in gingival fibroblasts. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Dan (Verified Customer) (02-27-2026) | Mouse serum samples from DNA therapy treated animals or normal controls
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