Mouse MFG-E8 ELISA Kit
Cat no : KE10062
Synonyms
AA408458, AI325141, lactadherin, MFG E8, Mfge8, Mfgm, P47, SED1
Validation Data Gallery
Product Information
KE10062 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MFG-E8 ELISA kit is to be used to detect and quantify protein levels of endogenous MFG-E8. The assay recognizes mouse MFG-E8. An antibody specific for MFG-E8 has been pre-coated onto the microwells. The MFG-E8 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse MFG-E8 is added to detect the captured mouse MFG-E8 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse MFG-E8 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plamsa, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 8.3 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 17304 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 115% | 71%-135% |
| Cell culture supernatants | 109% | 93%-125% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,670.7 | 113.5 | 6.8 |
| 2 | 20 | 608.0 | 55.2 | 9.1 |
| 3 | 20 | 422.6 | 27.1 | 6.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,399.8 | 133.7 | 9.6 |
| 2 | 24 | 621.0 | 56.7 | 9.1 |
| 3 | 24 | 421.2 | 38.8 | 9.2 |
Background Information
Milk fat globule-epidermal growth factor-factor 8 (MFG-E8), also known as lactadherin, is a multifunctional secreted glycoprotein exhibiting versatile functions in cell physiology affecting health and diseases. It plays roles in clearance of apoptotic cells, anti-inflammatory, wound healing, arterial remodeling, angiogenesis, enhancing tumorigenicity and cancer metastasis. MFG-E8 consists of two-repeated EGF-likedomains, a mucin-like domain and two-repeated discoidin-like domains (C-domains), and contains an integrin-binding motif (RGD sequence) in the EGF-like domain. It shows ubiquitous pattern of expression in various types of cells and tissues. Altered expression of MFG-E8 causes disruptions of homeostasis and is correlated with a number of diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AA408458, AI325141, lactadherin, MFG E8, Mfge8, Mfgm, P47, SED1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.