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Mouse MMP12 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: mouse

Detection: mouse

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.469-60 ng/mL

Sandwich ELISA: 78.1-5000 pg/mL

Cat no : MP03377-1

Synonyms

EC:3.4.24.65, Macrophage metalloelastase, Matrix metalloproteinase-12, MME, Mmel

Validation Data Gallery

  • Sandwich ELISA standard curve of MP03377-1, Mouse MMP12 Recombinant Matched Antibody Pair - PBS only. 88215-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3172. 88215-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

    Sandwich ELISA standard curve of MP03377-1

    Sandwich ELISA standard curve of MP03377-1, Mouse MMP12 Recombinant Matched Antibody Pair - PBS only. 88215-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3172. 88215-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • The mean MMP12 concentration was determined to be 81.7 ng/mL in mouse lung supernatant and 6.2 ng/mL in mouse spleen supernatant.

    Sandwich ELISA sample test of MP03377-1

    The mean MMP12 concentration was determined to be 81.7 ng/mL in mouse lung supernatant and 6.2 ng/mL in mouse spleen supernatant.

  • The mean MMP12 concentration was determined to be 8.1 ng/mL in mouse lung tissue extract based on a 5.0 mg/mL extract load.

    Sandwich ELISA sample test of MP03377-1

    The mean MMP12 concentration was determined to be 8.1 ng/mL in mouse lung tissue extract based on a 5.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP03377-1, MOUSE MMP12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88215-2-PBS. Detection antibody: 88215-1-PBS. Standard: Eg3172. Range: 0.469-60 ng/mL.

    Cytometric bead array standard curve of MP03377-1

    Cytometric bead array standard curve of MP03377-1, MOUSE MMP12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88215-2-PBS. Detection antibody: 88215-1-PBS. Standard: Eg3172. Range: 0.469-60 ng/mL.

  • The mean MMP12 concentration was determined to be 21.0 ng/mL in mouse lung supernatant and 4.1 ng/mL in mouse spleen supernatant.

    Cytometric bead array sample test of MP03377-1

    The mean MMP12 concentration was determined to be 21.0 ng/mL in mouse lung supernatant and 4.1 ng/mL in mouse spleen supernatant.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP03377-1 targets MMP12 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.469-60 ng/mL (Cytometric Bead Array); 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: Mouse Mmp12 Recombinant monoclonal antibody, PBS Only (Capture) 88215-2-PBS (260147A3). 100 μg. Concentration 1 mg/ml.

Detection antibody: Mouse Mmp12 Recombinant monoclonal antibody, PBS Only (Detector) 88215-1-PBS (260147D4). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: Eg3172

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 88215-2-PBS
Tested Reactivity mouse
Immunogen Mmp12 fusion protein Eg3172
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name matrix metallopeptidase 12
Synonyms EC:3.4.24.65, Macrophage metalloelastase, Matrix metalloproteinase-12, MME, Mmel
GenBank accession numberNM_008605.3
Gene symbol Mmp12
UniprotP34960
Gene ID (NCBI) 17381
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 88215-1-PBS
Tested Reactivity mouse
Immunogen Mmp12 fusion protein Eg3172
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name matrix metallopeptidase 12
Synonyms EC:3.4.24.65, Macrophage metalloelastase, Matrix metalloproteinase-12, MME, Mmel
GenBank accession numberNM_008605.3
Gene symbol Mmp12
UniprotP34960
Gene ID (NCBI) 17381
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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