Mouse Mesothelin ELISA Kit
Cat no : KE10222
Synonyms
Megakaryocyte-potentiating factor, Mes, Mesothelin, cleaved form, Mpf, Msln
Validation Data Gallery
Product Information
KE10222 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Mesothelin ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Mesothelin. The assay recognizes mouse Mesothelin. An antibody specific for mouse Mesothelin has been pre-coated onto the microwells. The mouse Mesothelin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse Mesothelin is added to detect the captured mouse Mesothelin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Mesothelin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 56047 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 97% | 90%-106% |
| Cell lysate | 91% | 81%-100% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 10.02 | 0.76 | 7.58 |
| 2 | 8 | 2.48 | 0.18 | 7.24 |
| 3 | 8 | 0.64 | 0.05 | 8.03 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 10.50 | 1.08 | 10.33 |
| 2 | 16 | 2.58 | 0.26 | 10.19 |
| 3 | 16 | 0.68 | 0.08 | 12.54 |
Background Information
Mesothelin is a 40-kDa cell surface glycoprotein that is present on normal mesothelial cells lining the pleura, peritoneum and pericardium. Mesothelin expression in normal human tissues is observed only in a single layer of mesothelial cells lining the pleura, peritoneum and pericardium, surface epithelial cells of the ovary, tunica vaginalis, rete testis and the tonsilar and fallopian tube epithelial cells. However, mesothelin is highly expressed in several cancers, including epitheloid mesotheliomas, pancreatic, biliary adenocarcinomas, gastric and ovarian cancers.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Megakaryocyte-potentiating factor, Mes, Mesothelin, cleaved form, Mpf, Msln |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.