Mouse PCSK9 ELISA Kit
Cat no : KE10050
Synonyms
AI415265, AI747682, FH3, HCHOLA3, Narc1, Pcsk9
Validation Data Gallery
Product Information
KE10050 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PCSK9 ELISA kit is to be used to detect and quantify protein levels of endogenous PCSK9. The assay recognizes mouse PCSK9. An antibody specific for PCSK9 has been pre-coated onto the microwells. The PCSK9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for PCSK9 is added to detect the captured PCSK9 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse PCSK9 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plamsa, Cell culture supernatants, Tissue lysates |
| Assay type | Sandwich |
| Sensitivity | 11.9 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 100102 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 104% | 86%-125% |
| Cell culture supernatants | 89% | 80%-103% |
| Tissue lysates | 86% | 78%-93% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,867.8 | 84.8 | 4.5 |
| 2 | 20 | 440.0 | 14.1 | 3.2 |
| 3 | 20 | 99.8 | 5.2 | 5.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,101.9 | 113.6 | 5.4 |
| 2 | 24 | 474.5 | 24.8 | 5.2 |
| 3 | 24 | 111.6 | 3.9 | 3.5 |
Background Information
PCSK9, also named as NARC1 and PC9, belongs to the peptidase S8 family. PCSK9 is expressed and secreted by several tissues but principally by the liver, small intestines, and kidney(PMID 28587771). It may be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. PCSK9 is a regulator of the LDL receptor and plasma cholesterol concentrations(PMID: 26293463).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AI415265, AI747682, FH3, HCHOLA3, Narc1, Pcsk9 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
J Nanobiotechnology Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapy | ||
Cancer Commun (Lond) Lipid metabolism reprograming by SREBP1-PCSK9 targeting sensitizes pancreatic cancer to immunochemotherapy | ||
Mol Ther Engineered extracellular vesicles as nanosponges for lysosomal degradation of PCSK9 | ||
Neoplasia L-methionine promotes CD8+ T cells killing hepatocellular carcinoma by inhibiting NR1I2/PCSK9 signaling | ||
Nat Commun Protein-nucleic acid language model-assisted design of precise and compact adenine base editor. |