Mouse PROCR ELISA Kit
Cat no : KE10149
Synonyms
Activated protein C receptor, APC receptor, CD201, Centrocyclin, Centrosomal protein CCD41
Validation Data Gallery
Product Information
KE10149 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse PROCR ELISA kit is to be used to detect and quantify protein levels of endogenous mouse PROCR. The assay recognizes mouse PROCR. An antibody specific for mouse PROCR has been pre-coated onto the microwells. The mouse PROCR protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse PROCR is added to detect the captured mouse PROCR protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse PROCR ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 5.8 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 19124 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 116% | 107%-123% |
| Tissue lysate | 111% | 107%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,245.0 | 58.7 | 4.7 |
| 2 | 8 | 313.1 | 9.7 | 3.1 |
| 3 | 8 | 94.8 | 3.1 | 3.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,226.7 | 52.5 | 4.3 |
| 2 | 16 | 311.1 | 12.6 | 4.1 |
| 3 | 16 | 94.6 | 3.7 | 3.9 |
Background Information
Endothelial protein C receptor (PROCR, also known as CD201 and EPCR) is a type I transmembrane glycoprotein that plays a critical role in the protein C anticoagulant pathway. Soluble PROCR (sPROCR) and PROCR bind Protein C (PC) and activated PC (APC) with similar affinity and the binding of sPROCR to APC inhibits its anticoagulant activity. Levels of sPROCR were associated with PC levels in the Leiden Thrombophilia Study (LETS). Additionally, PROCR has been implicated in stem cell maintenance and cancer biology, where it may influence cell proliferation and survival.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Activated protein C receptor, APC receptor, CD201, Centrocyclin, Centrosomal protein CCD41 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.