Mouse Pentraxin 3/TSG-14 ELISA Kit
Cat no : KE10073
Synonyms
AI607804, pentraxin related gene, Ptx3, TSG 14
Validation Data Gallery
Product Information
KE10073 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse Pentraxin 3/TSG-14 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Pentraxin 3/TSG-14. The assay recognizes mouse Pentraxin 3/TSG-14. An antibody specific for mouse Pentraxin 3/TSG-14 has been pre-coated onto the microwells. The mouse Pentraxin 3/TSG-14 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse Pentraxin 3/TSG-14 is added to detect the captured mouse Pentraxin 3/TSG-14 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Pentraxin 3/TSG-14 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 8.8 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 19288 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 89% | 70%-124% |
| Cell culture supernatants | 94% | 76%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 3,981.3 | 122.7 | 3.1 |
| 2 | 20 | 994.3 | 24.4 | 2.5 |
| 3 | 20 | 229.8 | 5.9 | 2.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 3,862.4 | 74.1 | 1.9 |
| 2 | 24 | 982.2 | 20.8 | 2.1 |
| 3 | 24 | 238.9 | 4.5 | 1.9 |
Background Information
Pentraxin 3 (PTX3), also known as TNF-inducible gene 14 protein (TSG-14), is a member of the pentraxin superfamily which consists of evolutionarily conserved proteins characterized by a structural motif, the pentraxin domain. Pentraxin 3 can be produced by a variety of cell types including endothelial cells, smooth muscle cells, adipocytes, fibroblasts, mononuclear phagocytes, and dendritic cells, and its expression is induced by primary inflammatory signals, such as IL-1, TNF, and microbial moieties. Pentraxin 3 is an acute phase glycoprotein that plays a role in the regulation of innate resistance to pathogens, inflammation, tissue remodeling and repair, female fertility and cancer. Pentraxin 3 is a biomarker for cardiovascular disease. Increased plasma Pentraxin 3 levels have been found in patients with cardiovascular disorders.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AI607804, pentraxin related gene, Ptx3, TSG 14 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.