Speedy™ Mouse/Rat HGF One-Step ELISA Kit
Cat no : SE60014
Synonyms
Hepatocyte growth factor, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain, Hepatopoietin-A, Scatter factor
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse/Rat HGF One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Tissue homogenates |
| Assay type | Sandwich |
| Sensitivity | 7.8 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 15234 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 124% | 122%-128% |
| Rat serum | 121% | 115%-126% |
| Cell culture supernatant | 107% | 88%-127% |
| Tissue homogenate | 120% | 116%-128% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,032.5 | 38.5 | 1.9 |
| 2 | 8 | 518.4 | 13.2 | 2.5 |
| 3 | 8 | 257.8 | 5.5 | 2.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,074.0 | 103.6 | 5.0 |
| 2 | 16 | 518.2 | 9.7 | 1.9 |
| 3 | 16 | 256.1 | 7.0 | 2.7 |
Background Information
HGF (hepatocyte growth factor) is a potent mitogen for hepatocytes, also known as hepatopoietin A (HBGA). HGF and its receptor MET are involved in cell proliferation, movement, differentiation, invasion, angiogenesis, and apoptosis by activating multiple downstream signaling pathways. HGF/cMet also can serve as biomarkers for gastrointestinal cancers. HGF regulates inflammation including collagen-induced arthritis, autoimmune neuroinflammation, inflammatory bowel disease and airway inflammation. Elevated levels of HGF of tumors and plasma were observed such as in invasive breast carcinoma, glioma, multiple myeloma and sarcomas.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Hepatocyte growth factor, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain, Hepatopoietin-A, Scatter factor |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.