Mouse/Rat IGF1 ELISA Kit
Cat no : KE20037
Synonyms
Igf-1, IGF-I, Insulin-like growth factor I, Somatomedin
Validation Data Gallery
Product Information
KE20037 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse/rat IGF1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat IGF1. The assay recognizes mouse/rat IGF1. An antibody specific for mouse/rat IGF1 has been pre-coated onto the microwells. The mouse/rat IGF1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse/rat IGF1 is added to detect the captured mouse/rat IGF1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse/Rat IGF1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Tissue lysates |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 1.95-125 pg/mL |
| Reactivity | Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 24482 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 99% | 93%-106% |
| Rat serum | 89% | 87%-91% |
| Mouse tissue lysate | 95% | 81%-105% |
| Rat tissue lysate | 98% | 95%-101% |
| Cell culture supernatant | 98% | 86%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 60.8 | 2.2 | 3.6 |
| 2 | 8 | 16.0 | 0.4 | 2.4 |
| 3 | 8 | 8.2 | 0.2 | 2.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 62.6 | 2.8 | 4.5 |
| 2 | 16 | 16.5 | 0.7 | 4.0 |
| 3 | 16 | 8.4 | 0.3 | 3.8 |
Background Information
Insulin-like-growth factor 1 (IGF1), a 70 amino-acid peptide hormone, is the principal mediator of biochemical effects of growth hormone (GH) and important in the regulation of cell proliferation and differentiation. IGF1 is largely synthesized in the liver (75%) and, to a lesser extent, in peripheral tissues. IGF1 is a potent mitogen and is inhibited by IGF-binding protein-3 (IGFBP3). High serum IGF1 and low IGFBP3 are associated with increased risk of several carcinomas. Mice lacking IGF1 exhibit generalized organ hypoplasia including underdevelopment of the central nervous system and developmental defects in bone, muscle and reproductive systems.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Igf-1, IGF-I, Insulin-like growth factor I, Somatomedin |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Phys Act Nutr Effects of rice protein hydrolysate on bone fracture properties in ovariectomized rats |