Mouse/Rat RBP4 ELISA Kit
Cat no : KE10197
Synonyms
Plasma retinol-binding protein, PRBP, RBP, Retinol-binding protein 4
Validation Data Gallery
Product Information
KE10197 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse/rat RBP4 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat RBP4. The assay recognizes mouse/rat RBP4. An antibody specific for mouse/rat RBP4 has been pre-coated onto the microwells. The mouse/rat RBP4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse/rat RBP4 is added to detect the captured mouse/rat RBP4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse/Rat RBP4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine, Tissue lysates |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.313-10 ng/mL |
| Reactivity | Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 19662 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 94% | 86%-99% |
| Rat serum | 99% | 95%-103% |
| Mouse urine | 81% | 73%-88% |
| Cell culture supernatant | 84% | 80%-87% |
| Tissue lysate | 88% | 80%-98% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.69 | 0.22 | 4.68 |
| 2 | 8 | 1.57 | 0.07 | 4.50 |
| 3 | 8 | 0.85 | 0.05 | 5.42 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.80 | 0.21 | 4.38 |
| 2 | 16 | 1.52 | 0.08 | 5.46 |
| 3 | 16 | 0.84 | 0.06 | 7.67 |
Background Information
RBP4 (Retinol Binding Protein 4) is a soluble protein belonging to the lipoprotein family, primarily expressed in the liver and responsible for transporting retinol (vitamin A) in the bloodstream. After binding to retinol, RBP4 forms a complex with transthyretin (TTR) and transports retinol to tissues throughout the body via the circulatory system. In target tissues, RBP4 delivers retinol into cells through the membrane receptor STRA6. RBP4 not only plays a key role in retinol metabolism but is also associated with various physiological and pathological processes. It can induce the expression of pro-inflammatory cytokines in macrophages, participate in inflammatory responses, and affect insulin resistance by activating the JNK and TLR4 signaling pathways. Additionally, RBP4 undergoes filtration and reabsorption in the kidneys, and an increase in urinary RBP4 levels serves as a strong indicator for diagnosing proximal tubular dysfunction. RBP4 may also transport other molecules, such as certain fatty acids, and is involved in fatty acid metabolism.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Plasma retinol-binding protein, PRBP, RBP, Retinol-binding protein 4 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.