Mouse/Rat TREM-1 ELISA Kit
Cat no : KE10080
Synonyms
Trem1
Validation Data Gallery
Product Information
KE10080 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse/rat TREM-1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat TREM-1. The assay recognizes mouse/rat TREM-1. An antibody specific for mouse/rat TREM-1 has been pre-coated onto the microwells. The mouse/rat TREM-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse/rat TREM-1 is added to detect the captured mouse/rat TREM-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse/Rat TREM-1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Tissue lysates |
| Assay type | Sandwich |
| Sensitivity | 0.4 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 58217 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 92% | 79%-108% |
| Mouse serum | 88% | 70%-130% |
| Rat serum | 86% | 70%-118% |
| Tissue lysates | 115% | 99%-125% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 443.0 | 16.8 | 3.8 |
| 2 | 20 | 104.4 | 2.2 | 2.1 |
| 3 | 20 | 24.4 | 1.4 | 5.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 428.5 | 13.0 | 3.0 |
| 2 | 24 | 102.8 | 2.0 | 1.9 |
| 3 | 24 | 26.5 | 1.0 | 3.7 |
Background Information
Triggering receptor expressed on myeloid cells 1 (TREM-1, also known as CD354) is a member of the immunoglobulin superfamily, which is expressed on neutrophils and monocytes. TREM1 plays a role in the innate immune system. TREM1 amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. TREM1 can also exist as a soluble form (sTREM1). Increased plasma level of sTREM1 have been reported in specific diseases including Alzheimer's disease, systemic sepsis, acute myocardial infarction and subarachnoid hemorrhage.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Trem1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.