Mouse Resistin ELISA Kit
Cat no : KE10055
Synonyms
Adipose tissue-specific secretory factor, Adipose-specific cysteine-rich secreted protein A12-alpha, ADSF, Cysteine-rich secreted protein FIZZ3, Fizz3
Validation Data Gallery
Product Information
KE10055 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Resistin ELISA kit is to be used to detect and quantify protein levels of endogenous Resistin. The assay recognizes mouse Resistin. An antibody specific for Resistin has been pre-coated onto the microwells. The Resistin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Resistin is added to detect the captured Resistin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Resistin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 57264 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 103% | 91%-121% |
| Cell culture supernatants | 95% | 83%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 255.1 | 4.9 | 1.9 |
| 2 | 20 | 59.1 | 1.2 | 2.0 |
| 3 | 20 | 11.6 | 0.7 | 6.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 242.2 | 9.3 | 3.8 |
| 2 | 24 | 57.7 | 2.1 | 3.7 |
| 3 | 24 | 14.0 | 1.0 | 6.9 |
Background Information
Resistin (or 'resistance to insulin') was originally discovered in mice in 2001 and named for its ability to resist (interfere with) insulin action; at that time, it was proposed as a link between obesity and diabetes (PMID: 11201732). Mouse resistin is an 11 kDa cysteine-rich polypeptide, and its gene is located on chromosome 8. It is synthesized as a 114 amino acid precursor, with a 20 aa signal sequence and a 94 aa mature segment (PMID: 21545576). Neuroprotective nature of adipokine resistin in the early stages of focal cerebral ischemia in a stroke mouse model suggests that resistin may have a therapeutic potential effect in management of neurodegenerative disease (PMID: 29408288).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Adipose tissue-specific secretory factor, Adipose-specific cysteine-rich secreted protein A12-alpha, ADSF, Cysteine-rich secreted protein FIZZ3, Fizz3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.