Mouse SIRP Alpha/CD172a ELISA Kit
Cat no : KE10186
Synonyms
Bit, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a, Inhibitory receptor SHPS-1
Validation Data Gallery
Product Information
KE10186 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse SIRP Alpha/CD172a ELISA kit is to be used to detect and quantify protein levels of endogenous mouse SIRP Alpha/CD172a. The assay recognizes mouse SIRP Alpha/CD172a. An antibody specific for mouse SIRP Alpha/CD172a has been pre-coated onto the microwells. The mouse SIRP Alpha/CD172a protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse SIRP Alpha/CD172a is added to detect the captured mouse SIRP Alpha/CD172a protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse SIRP Alpha/CD172a ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate, Tissue lysate, Tissue homogenates |
| Assay type | Sandwich |
| Sensitivity | 0.3 pg/mL |
| Range | 23.4-1500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 19261 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 91% | 83%-96% |
| Lysate | 92% | 89%-94% |
| Tissue homogenates | 89% | 80%-95% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 747.0 | 32.3 | 4.3 |
| 2 | 8 | 197.2 | 12.3 | 6.2 |
| 3 | 8 | 100.3 | 3.2 | 3.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 735.5 | 28.6 | 3.9 |
| 2 | 16 | 196.9 | 11.5 | 5.9 |
| 3 | 16 | 106.7 | 7.1 | 6.7 |
Background Information
Signal-regulatory protein alpha (SIRP Alpha, also known as CD172a) is a transmembrane glycoprotein that belongs to the SIRP family. It is expressed primarily on myeloid cells and acts as an inhibitory receptor. SIRP Alpha interacts with CD47, also known as the "do not eat me" signal, to inhibit the phagocytosis of normal cells by macrophages. This interaction is crucial for preventing the immune system from attacking healthy cells. Additionally, it also participates in the negative regulation of receptor tyrosine kinase signaling pathways and mediates the negative regulation of phagocytosis, mast cell activation, and dendritic cell activation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Bit, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a, Inhibitory receptor SHPS-1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.