Mouse TCB1 ELISA Kit
Cat no : KE10195
Synonyms
Validation Data Gallery
Product Information
KE10195 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse TCB1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse TCB1. The assay recognizes mouse TCB1. An antibody specific for mouse TCB1 has been pre-coated onto the microwells. The mouse TCB1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse TCB1 is added to detect the captured mouse TCB1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 97% | 93%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,249.6 | 58.8 | 4.7 |
| 2 | 8 | 323.7 | 28.4 | 8.8 |
| 3 | 8 | 185.7 | 10.5 | 5.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,282.4 | 102.3 | 8.0 |
| 2 | 16 | 324.9 | 21.5 | 6.6 |
| 3 | 16 | 176.4 | 12.5 | 7.1 |
Background Information
T-cell receptor beta-1 chain C region (TCB1) is a critical component of the TCR complex, which is essential for T-cell antigen recognition and activation. The TCR complex consists of the highly variable alpha (α) and beta (β) chains, along with invariant CD3 chain molecules. The beta-1 chain C region provides structural stability and is involved in signal transduction upon antigen recognition. This region is crucial for the proper assembly and function of the TCR complex, enabling T cells to recognize and respond to specific antigens presented by major histocompatibility complex (MHC) molecules.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.