Mouse TNFRSF9/CD137 ELISA Kit
Cat no : KE10181
Synonyms
4-1BB, 4-1BB ligand receptor, CD137, Ila, Ly63
Validation Data Gallery
Product Information
KE10181 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse TNFRSF9/CD137 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse TNFRSF9/CD137. The assay recognizes mouse TNFRSF9/CD137. An antibody specific for mouse TNFRSF9/CD137 has been pre-coated onto the microwells. The mouse TNFRSF9/CD137 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse TNFRSF9/CD137 is added to detect the captured mouse TNFRSF9/CD137 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse TNFRSF9/CD137 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 21942 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 101% | 97%-110% |
| Cell lysate | 109% | 104%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 982.8 | 64.2 | 6.5 |
| 2 | 8 | 205.9 | 9.5 | 4.6 |
| 3 | 8 | 103.2 | 5.9 | 5.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 923.9 | 77.1 | 8.3 |
| 2 | 16 | 206.4 | 8.6 | 4.2 |
| 3 | 16 | 109.5 | 9.3 | 8.5 |
Background Information
Tumor necrosis factor receptor superfamily member 9 (TNFRSF9, also known as CD137 and 4-1BB) is a member of the TNF receptor superfamily. It is an inducible T cell surface receptor that plays a crucial role in the development and activation of multiple immune cells. The interaction of CD137 with its ligand, 4-1BBL, costimulates T cell proliferation, activation, and survival, and can enhance activation-induced cell death of repetitively stimulated T cells. This receptor also contributes to the development of CD8 cytotoxic T cells and anti-tumor immunity. Moreover, soluble CD137 (sCD137) protein levels were elevated in adipose tissues of obese subjects, and sCD137 enhanced obesity-induced adipose inflammation by triggering CD137L reverse inflammatory signals in macrophages and activated T cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 4-1BB, 4-1BB ligand receptor, CD137, Ila, Ly63 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.