Mouse TSLP ELISA Kit
Cat no : KE10083
Synonyms
thymic stromal lymphopoietin, Tslp
Validation Data Gallery
Product Information
KE10083 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse TSLP ELISA kit is to be used to detect and quantify protein levels of endogenous mouse TSLP. The assay recognizes mouse TSLP. An antibody specific for mouse TSLP has been pre-coated onto the microwells. The mouse TSLP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse TSLP is added to detect the captured mouse TSLP protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
| Product name | Mouse TSLP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.3 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 53603 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 86% | 73%-115% |
| Cell culture supernatants | 102% | 75%-123% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 118.7 | 6.7 | 5.6 |
| 2 | 20 | 207.8 | 12.1 | 5.8 |
| 3 | 20 | 376.4 | 11.0 | 2.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 118.9 | 7.0 | 5.9 |
| 2 | 24 | 204.2 | 12.0 | 5.9 |
| 3 | 24 | 355.4 | 32.3 | 9.1 |
Background Information
Thymic stromal lymphopoietin (TSLP) is a 24 kDa monomeric glycoprotein that was originally identified as a product of a thymic stromal cell line. TSLP mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c+ dendritic cells. It can induce allergic inflammation by directly activating mast cells. TSLP is expressed in a number of tissues including heart, liver and prostate. Human TSLP shares only 38% aa sequence identity with mouse TSLP in the mature region. TSLP has been shown to expand the CD4+ subset of lymphocytes and thymocytes and thus regulate CD4+ homeostasis. Overexpression of TSLP causes imbalances in lymphopoiesis and myelopoiesis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | thymic stromal lymphopoietin, Tslp |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Immunol Lett Role of G-protein-coupled estrogen receptor in the pathogenesis of chronic asthma |