Mouse TWEAK R/TNFRSF12 ELISA Kit
Cat no : KE10199
Synonyms
TNFRSF12, TWEAK R, CD266, FGF-inducible 14, Fgfrp2
Validation Data Gallery
Product Information
KE10199 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse TWEAK R/TNFRSF12 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse TWEAK R/TNFRSF12. The assay recognizes mouse TWEAK R/TNFRSF12. An antibody specific for mouse TWEAK R/TNFRSF12 has been pre-coated onto the microwells. The mouse TWEAK R/TNFRSF12 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse TWEAK R/TNFRSF12 is added to detect the captured mouse TWEAK R/TNFRSF12 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse TWEAK R/TNFRSF12 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 4.9 pg/mL |
| Range | 19.5-1250 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 27279 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 93% | 72%-122% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 646.4 | 44.5 | 6.9 |
| 2 | 8 | 116.9 | 6.3 | 5.4 |
| 3 | 8 | 23.7 | 2.5 | 10.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 664.8 | 44.1 | 6.6 |
| 2 | 16 | 122.4 | 8.2 | 6.7 |
| 3 | 16 | 25.7 | 3.3 | 12.9 |
Background Information
Tumor necrosis factor receptor superfamily member 12 (TNFRSF12, also known as TWEAK R) is a type I transmembrane protein and a member of the tumor necrosis factor receptor (TNFR) superfamily. It acts as a receptor for TNFSF12/TWEAK and plays a crucial role in immune system development and function. TNFRSF12 was reported to contribute to microvascular permeability in cerebral ischemia, as well as pathologic angiogenesis in tumors. Additionally, it may also be associated with the progression of fibrotic kidney disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TNFRSF12, TWEAK R, CD266, FGF-inducible 14, Fgfrp2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.