Mouse Thrombopoietin ELISA Kit
Cat no : KE10059
Synonyms
C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, MGDF, Myeloproliferative leukemia virus oncogene ligand, Thrombopoietin, Tpo
Validation Data Gallery
Product Information
KE10059 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Thrombopoietin ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Thrombopoietin. The assay recognizes mouse Thrombopoietin. An antibody specific for mouse Thrombopoietin has been pre-coated onto the microwells. The mouse Thrombopoietin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse Thrombopoietin is added to detect the captured mouse Thrombopoietin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Thrombopoietin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 8.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 21832 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 85% | 78%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,961.9 | 55.0 | 2.8 |
| 2 | 20 | 467.5 | 13.5 | 2.9 |
| 3 | 20 | 92.0 | 5.0 | 5.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,891.6 | 42.2 | 2.2 |
| 2 | 24 | 457.5 | 11.5 | 2.5 |
| 3 | 24 | 110.5 | 4.4 | 3.9 |
Background Information
Thrombopoietin (TPO) is produced in the liver by both parenchymal cells and sinusoidal endothelial cells, as well as in the kidney by proximal convoluted tubule cells. It is a primary regulator of megakaryocyte development and platelet production in mammals. TPO binds the TPO receptor, activates JAK and STAT pathways, thus stimulating megakaryocyte growth and platelet production. Additionally, as TPO is vital for the maintenance of haematopoietic stem cells, it can truly be described as a pan- haematopoietic cytokine.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, MGDF, Myeloproliferative leukemia virus oncogene ligand, Thrombopoietin, Tpo |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Nat Commun Histone lysine methyltransferases MLL3 and MLL4 direct gene expression to produce platelets efficiently. |