Mouse Tie-2 ELISA Kit
Cat no : KE10194
Synonyms
Angiopoietin-1 receptor, CD202b, EC:2.7.10.1, Endothelial tyrosine kinase, HYK
Validation Data Gallery
Product Information
KE10194 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Tie-2 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Tie-2. The assay recognizes mouse Tie-2. An antibody specific for mouse Tie-2 has been pre-coated onto the microwells. The mouse Tie-2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse Tie-2 is added to detect the captured mouse Tie-2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Tie-2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Cell lysate, Tissue lysate, Tissue homogenates |
| Assay type | Sandwich |
| Sensitivity | 7.6 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 21687 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 96% | 90%-109% |
| Cell culture supernatant | 96% | 94%-100% |
| Cell lysate | 101% | 97%-108% |
| Tissue homogenates | 103% | 93%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,574.2 | 52.4 | 3.3 |
| 2 | 8 | 376.5 | 20.1 | 5.3 |
| 3 | 8 | 194.1 | 8.1 | 4.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,500.7 | 103.6 | 6.9 |
| 2 | 16 | 408.6 | 37.2 | 9.1 |
| 3 | 16 | 218.0 | 28.7 | 13.2 |
Background Information
Tie-2 (also known as TEK or CD202b) is a receptor tyrosine kinase (RTK) critical for vascular development, angiogenesis, and endothelial cell function. Tie-2 is essential for blood vessel maturation. Genetic knockout of Tie-2 or Ang-1 in mice leads to embryonic lethality due to defects in cardiac development and vascular remodeling. Ligand-induced Tie-2 phosphorylation activates PI3K-AKT (promoting survival) and MAPK pathways (regulating proliferation and migration).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Angiopoietin-1 receptor, CD202b, EC:2.7.10.1, Endothelial tyrosine kinase, HYK |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.