Mouse Tnfrsf11b/Osteoprotegerin ELISA Kit
Cat no : KE10107
Synonyms
Tnfrsf11b,OCIF,Opg,Osteoprotegerin,PDB5
Validation Data Gallery
Product Information
KE10107 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Tnfrsf11b ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Tnfrsf11b. The assay recognizes mouse Tnfrsf11b. An antibody specific for mouse Tnfrsf11b has been pre-coated onto the microwells. The mouse Tnfrsf11b protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse Tnfrsf11b is added to detect the captured mouse Tnfrsf11b protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Tnfrsf11b/Osteoprotegerin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma，Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 18383 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 102% | 85%-116% |
| Cell culture supernatants | 99% | 87%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 534.4 | 40.7 | 7.6 |
| 2 | 20 | 128.6 | 5.0 | 3.9 |
| 3 | 20 | 33.8 | 1.8 | 5.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 520.2 | 36.4 | 7.0 |
| 2 | 24 | 127.8 | 8.7 | 6.8 |
| 3 | 24 | 33.1 | 2.6 | 7.9 |
Background Information
TNFRSF11B, also known as Osteoprotegerin (OPG) or OCIF, is a member of the tumor necrosis factor receptor super family (TNFRSF). It is a secreted glycoprotein that inhibits osteoclastogenesis and is involved in the regulation of bone density. Osteoprotegerin acts as decoy receptor for receptor activator of nuclear factor-kappa B ligand (RANKL) and neutralizes its function in osteoclastogenesis. Osteoprotegerin may function as a soluble decoy receptor for tumor necrosis factor (TNF)-related apoptosis inducing ligand (TRAIL) and inhibits TRAIL-induced apoptosis. TRAIL blocks osteoprotegerin mediated inhibition of osteoclastogenesis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Tnfrsf11b,OCIF,Opg,Osteoprotegerin,PDB5 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Cell Signal ED-71 ameliorates OVX-induced osteoporosis by regulating calcium homeostasis and SIRT1-mediated mitochondrial function, alleviating osteoblast senescence and suppressing osteoclastogenesis |