Mouse Total MMP-3 ELISA Kit
Cat no : KE10040
Synonyms
EC:3.4.24.17, EMS-2, Matrix metalloproteinase-3, Mmp3, MMP-3
Validation Data Gallery
Product Information
KE10040 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Total MMP3 ELISA kit is to be used to detect and quantify protein levels of endogenous Total MMP3. The assay recognizes mouse Total MMP3. An antibody specific for Total MMP3 has been pre-coated onto the microwells. The Total MMP3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse Total MMP3 is added to detect the captured mouse Total MMP3 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Total MMP-3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 134.9 pg/mL |
| Range | 250-4000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 17392 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 106% | 96%-122% |
| Cell culture supernatants | 90% | 73%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 477.6 | 11.1 | 2.3 |
| 2 | 20 | 967.8 | 18.3 | 1.9 |
| 3 | 20 | 1,875.2 | 56.5 | 3.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 490.1 | 12.4 | 2.5 |
| 2 | 24 | 992.3 | 20.7 | 2.1 |
| 3 | 24 | 1,925.9 | 47.5 | 2.5 |
Background Information
MMP-3 (matrix metallopeptidase 3), also named as Stromelysin 1 or STR1, is a member of matrix metalloproteinase (MMP) family. The MMP family of enzymes is comprised of critically important extracellular matrix remodeling proteases whose activity has been implicated in normal embryogenesis and tissue remodelling and in many diseases such as arthritis, cancer, periodontitis, glomerulonephritis, encephalomyelitis, atherosclerosis and tissue ulceration. These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 is secreted from the cells as a proenzyme. The proenzyme has been shown to stimulate plasminogen activation. The active MMP-3 is capable of cleaving types III, IV, IX and X collagen, aggrecan, fbronectin, laminin, IGFBP-3, serpins, and IL-1β.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:3.4.24.17, EMS-2, Matrix metalloproteinase-3, Mmp3, MMP-3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
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|---|---|---|
PLoS Negl Trop Dis Dengue virus infection induces selective expansion of Vγ4 and Vγ6TCR γδ T cells in the small intestine and a cytokine storm driving vascular leakage in mice | ||
Cartilage miR-708-5p Attenuates Osteoarthritis Progression via Multi-Target Modulation of the NOX4/NF-κB Axis and Cartilage Homeostasis | ||
Int Immunopharmacol Sodium Houttuyfonate improves cognitive impairment in mice after chronic sleep deprivation by inhibiting microglial inflammation and ferroptosis. |