Mouse VEGF ELISA Kit

Sensitivity

2.0 pg/mL

Range

7.8-500 pg/mL

Reactivity

Mouse

Cat no : KE10009

Synonyms

Vegf, Vegf a, Vegf120, Vegf164, Vegf188, Vegfa, Vpf



Product Information

KE10009 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse VEGF ELISA kit is to be used to detect and quantify protein levels of endogenous mouse VEGF. The assay recognizes mouse VEGF. An antibody specific for mouse VEGF has been pre-coated onto the microwells. The mouse VEGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse VEGF is added to detect the captured mouse VEGF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Mouse VEGF ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 2.0 pg/mL
Range 7.8-500 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Mouse serum93%74%-110%
Cell culture supernatants94%92%-98%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12037.02.53.4
220118.09.03.8
320369.063.68.6

InterAssay

Samplenmean ( pg/mL)SDCV%
12439.04.55.7
224128.07.12.8
324390.031.94.1

Background Information

Vascular endothelial growth factor (VEGF), is a signal protein produced by cells that stimulates vasculogenesis and angiogenesis. It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate such as in hypoxic conditions. Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus. The activities of VEGF are not limited to the vascular system; VEGF plays a role in normal physiological functions such as bone formation, hematopoiesis, wound healing, and development. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. VEGF is upregulated in many known tumors and its expression is correlated with tumor stage and progression.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Vegf, Vegf a, Vegf120, Vegf164, Vegf188, Vegfa, Vpf

Publications

SpeciesSample TypeTitle

Int J Mol Sci

Network Pharmacology-Based Investigation of Protective Mechanism of Aster tataricus on Lipopolysaccharide-Induced Acute Lung Injury.

Authors - Yijun Chen
mouseserum

Evid Based Complement Alternat Med

Combination of Ligusticum Chuanxiong and Radix Paeonia Promotes Angiogenesis in Ischemic Myocardium through Notch Signalling and Mobilization of Stem Cells.

Authors - Wei-Li Shi
mouseplasma

Cell Death Discov

Microtubule associated protein 4 (MAP4) phosphorylation reduces cardiac microvascular density through NLRP3-related pyroptosis.

Authors - Yan-Hai Feng
mouseserum

Endocrinology

Dietary intake regulates white adipose tissues angiogenesis via liver fibroblast growth factor 21 in male mice.

Authors - Lun Hua
mouseserum

Theranostics

Cardio-renal Exosomes in Myocardial Infarction Serum Regulate Proangiogenic Paracrine Signaling in Adipose Mesenchymal Stem Cells.

Authors - Lei Gao
mousecell culture supernatants

Nat Nanotechnol

Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades.

Authors - Xuefei Lu