Mouse VEGFD ELISA Kit
Cat no : KE10146
Synonyms
Figf,c fos induced growth factor,Vascular endothel
Validation Data Gallery
Product Information
KE10146 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The humanVEGFD ELISA kit is to be used to detect and quantify protein levels of endogenous humanVEGFD. The assay recognizes humanVEGFD. An antibody specific for humanVEGFD has been pre-coated onto the microwells. The humanVEGFD protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for humanVEGFD is added to detect the captured humanVEGFD protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse VEGFD ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 14205 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 93% | 87%-104% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.81 | 0.16 | 3.30 |
| 2 | 8 | 1.08 | 0.02 | 2.01 |
| 3 | 8 | 0.54 | 0.01 | 2.41 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.72 | 0.19 | 4.11 |
| 2 | 16 | 1.07 | 0.02 | 2.16 |
| 3 | 16 | 0.54 | 0.01 | 2.35 |
Background Information
Vascular Endothelial Growth Factor-D (VEGF-D) is a member of the Vascular Endothelial Growth Factor (VEGF) family, which plays a crucial role in both angiogenesis and lymphangiogenesis. In murine embryos, VEGF-D is expressed in various major organs, including the kidney, lungs, adrenal glands, and vertebral column. In healthy humans, it is expressed in the lungs, heart, skeletal muscle, colon, and small intestine. VEGF-D is involved in tumor angiogenesis and lymphangiogenesis. It can promote the growth of blood vessels and lymphatic vessels in tumors, facilitating the spread and metastasis of cancer cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Figf,c fos induced growth factor,Vascular endothel |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.