Mouse gp130/IL6ST ELISA Kit
Cat no : KE10212
Synonyms
CD130, gp130, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6RB
Validation Data Gallery
Product Information
KE10212 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse gp130/IL6ST ELISA kit is to be used to detect and quantify protein levels of endogenous mouse gp130/IL6ST. The assay recognizes mouse gp130/IL6ST. An antibody specific for mouse gp130/IL6ST has been pre-coated onto the microwells. The mouse gp130/IL6ST protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse gp130/IL6ST is added to detect the captured mouse gp130/IL6ST protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse gp130/IL6ST ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 28.4 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16195 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 96% | 72%-113% |
| Cell lysate | 92% | 85%-102% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,493.3 | 87.1 | 3.5 |
| 2 | 8 | 761.7 | 33.8 | 4.4 |
| 3 | 8 | 239.9 | 8.3 | 3.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,545.9 | 132.2 | 5.2 |
| 2 | 16 | 755.3 | 29.6 | 3.9 |
| 3 | 16 | 244.3 | 7.7 | 3.1 |
Background Information
GP130, also known as IL6ST, is a transmembrane protein that serves as a shared signal-transducing subunit for a variety of cytokines, including interleukin-6 (IL-6), IL-11, IL-27, leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine (CLC)/CLC factor 1 (CLCF-1), and neuropoietin. This protein is highly conserved among vertebrates and plays a crucial role in mediating interactions between a wide range of immune and non-immune cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD130, gp130, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6RB |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.