Mouse MPO Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP01284-4

  Sandwich ELISA standard curve of MP01284-4, Mouse MPO Recombinant Matched Antibody Pair - PBS only. 84436-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0702. 84436-4-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL

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  Sample test of MP01284-4

  Serum of eight mice was measured. The mouse MPO concentration of detected samples was determined to be 4.86 ng/mL with a range of 2.54 - 9.19 ng/mL.

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  IF Staining of mouse spleen using 84436-4-RR (same clone as 84436-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using MPO antibody (84436-4-RR, Clone: 241839F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84436-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84436-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84436-4-RR against Mouse Mpo were performed. The affinity constant is below 1 pM.

MPO Uni-rAb^® Recombinant Antibody for IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84436-4-PBS

Clone No.241839F3

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

241839F3, EC:1.11.2.2, Myeloperoxidase, Myeloperoxidase heavy chain, Myeloperoxidase light chain

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Product Information

84436-4-PBS targets MPO as part of a matched antibody pair:

MP01284-4: 84436-3-PBS capture and 84436-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0702

  Product name: Recombinant Mouse Myeloperoxidase protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-10*His

  Domain: 16-718 aa of AAR99349

  Sequence: MLQTSNGATPALLGEVENSVVLSCMEEAKQLVDRAYKERRESIKRSLQSGSASPTELLFYFKQPVAGTRTAVRAADYLHVALDLLKRKLQPLWPRPFNVTDVLTPAQLNLLSVSSGCAYQDVGVTCPPNDKYRTITGHCNNRRSPTLGASNRAFVRWLPAEYEDGVSMPFGWTPGVNRNGFKVPLARQVSNAIVRFPNDQLTKDQERALMFMQWGQFLDHDITLTPEPATRFSFFTGLNCETSCLQQPPCFPLKIPPNDPRIKNQKDCIPFFRSCPACTRNNITIRNQINALTSFVDASGVYGSEDPLARKLRNLTNQLGLLAVNTRFQDNGRALMPFDSLHDDPCLLTNRSARIPCFLAGDMRSSEMPELTSMHTLFVREHNRLATQLKRLNPRWNGEKLYQEARKIVGAMVQIITYRDYLPLVLGPAAMKKYLPQYRSYNDSVDPRIANVFTNAFRYGHTLIQPFMFRLNNQYRPTGPNPRVPLSKVFFASWRVVLEGGIDPILRGLMATPAKLNRQNQIVVDEIRERLFEQVMRIGLDLPALNMQRSRDHGLPGYNAWRRFCGLPQPSTVGELGTVLKNLELARKLMAQYGTPNNIDIWMGGVSEPLEPNGRVGQLLACLIGTQFRKLRDGDRFWWENPGVFSKQQRQALASISLPRIICDNTGITTVSKNNIFMSNTYPRDFVSCNTLPKLNLTSWKET

• Full Name: myeloperoxidase
• Calculated Molecular Weight: 81KD
• GenBank Accession Number: AAR99349
• Gene Symbol: Mpo
• Gene ID (NCBI): 17523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11247
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.