Multi-rAb™ CoraLite® Plus 555-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)

• 

  Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:800 and Multi-rAb™ CoraLite ® Plus 555-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM003).

• 

  Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, green) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, orange). Multi-rAb™ CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb™ CoraLite® Plus 555-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM003, 1:500) were used for detection.

• 

  Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, green) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, orange). Multi-rAb™ CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb™ CoraLite® Plus 555-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM003, 1:500) were used for detection.

• 

  Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, green) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, orange). Multi-rAb™ CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb™ CoraLite® Plus 555 conjugated Recombinant Goat anti-mouse secondary antibody were used for detection (RGAM003, 1:500).

• 

  Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, green) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, orange). Multi-rAb™ CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb™ CoraLite® Plus 555 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM003, 1:500) .

• 

  1X10^6 MOLT4 were surface stained with 0.2 ug Anti-Human CD8 (65204-1-Ig, Clone: UCHT4) and Mouse IgG2a Isotype Control 66360-2-Ig. Multi-rAb™ CoraLite® Plus 550-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM003 was used at 1:500 for detection.

Multi-rAb™ Goat Anti-Mouse Recombinant Secondary Antibody (H+L) is a mixture of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on the mouse IgG heavy and light chains.

Cat No. RGAM003

Publications

4

Applications

IF, FC

Conjugate

CoraLite® Plus 555

Host

Goat

Reactivity

Mouse

---

Product Information

RGAM003 targets Mouse IgG (H+L) in FC and IF. It can bind to all mouse IgG isotypes, mouse IgM and IgA as well as rat IgG. It shows minimal reactivity for rabbit, human, pig and bovine IgGs.

• Concentration: 0.5 mg/mL
• Applications: IF, FC
• Host Species: Goat
• Species Reactivity: Mouse
• Physical State: Liquid
• Suggested Dilution Range: 1:200-1:1000 for IF and FC
• Purity: The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads.
• Storage Buffer: PBS with 50% glycerol, 10 mg/mL BSA, 0.1% Proclin-300, pH 7.4.
• Storage Instructions: Store at -20°C. Stable for one year after shipment.
• Fluorophore: CoraLite® Plus 555, Amax=554 nm, Emax=570 nm
• Clonality: Multiclonal recombinant
• RRID: AB_3068539
• Note: This product is for research use only,not for diagnostic or therapeutic use.

Background

Multi-rAb™ Recombinant Secondary Antibodies are mixtures of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on the same IgG. Each recombinant clone in the final multiclonal mixture is carefully selected after rigorous characterization and screening to ensure the highest level of performance. ​The selected clones are mixed in various combinations, each of which is then functionally characterized in multiple applications to select the final multiclonal mixture with optimal performance. Each lot of Multi-rAb™ Secondary Antibodies is produced by mixing the same high performing recombinant clones every time to ensure that they have high batch-to-batch consistency, which in turn results in high reproducibility of experimental data.

Publications

Title
J Transl Med; HMGB1/TREM2 positive feedback loop drives the development of radioresistance and immune escape of glioblastoma by regulating TLR4/Akt signaling; Authors - Hui Qiu
Cell Biochem Funct; Matrix Stiffness Regulates Interleukin-10 Secretion in Human Microglia (HMC3) via YAP-Mediated Mechanotransduction; Authors - Xue Fang
Adv Sci (Weinh); PDZK1 Protects Against RPE Senescence by Targeting the 14-3-3ε-mTOR Axis to Attenuate Early Diabetic Retinopathy; Authors - Jian Zhao
J Transl Med; ATP11B triggers the infiltration of T cells into GBM and intensifies anti-GBM immunity by upregulating and externalizing S1PR1; Authors - Hui Qiu