Multi-rAb™ CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)

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  Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:800 and Multi-rAb™ CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

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  Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, green) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, red). Multi-rAb™ CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb™ CoraLite® Plus 594 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM004, 1:500) .

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  Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, green) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, red). Multi-rAb™ CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb™ CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004, 1:500) were used for detection.

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  Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, green) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, red). Multi-rAb™ CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb™ CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004, 1:500) were used for detection.

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  Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, green) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, red). Multi-rAb™ CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb™ CoraLite® Plus 594 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM004, 1:500) .

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  1X10^6 MOLT4 were surface stained with 0.2 ug Anti-Human CD8 (65204-1-Ig, Clone: UCHT4) and Mouse IgG2a Isotype Control 66360-2-Ig. Multi-rAb™ CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM004 was used at 1:500 for detection.

Multi-rAb™ Goat Anti-Mouse Recombinant Secondary Antibody (H+L) is a mixture of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on the mouse IgG heavy and light chains.

Cat No. RGAM004

Publications

31

Reviews (2)

Applications

IF, FC

Conjugate

CoraLite® Plus 594

Host

Goat

Reactivity

Mouse

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Product Information

RGAM004 targets Mouse IgG (H+L) in FC and IF. It can bind to all mouse IgG isotypes, mouse IgM and IgA as well as rat IgG. It shows minimal reactivity for rabbit, human, pig and bovine IgGs.

• Concentration: 0.5 mg/mL
• Applications: IF, FC
• Host Species: Goat
• Species Reactivity: Mouse
• Physical State: Liquid
• Suggested Dilution Range: 1:200-1:1000 for IF and FC
• Purity: The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads.
• Storage Buffer: PBS with 50% glycerol, 10 mg/mL BSA, 0.1% Proclin-300, pH 7.4.
• Storage Instructions: Store at -20°C. Stable for one year after shipment.
• Fluorophore: CoraLite® Plus 594, Amax=588 nm, Emax=604 nm
• Clonality: Multiclonal recombinant
• RRID: AB_3073502
• Note: This product is for research use only,not for diagnostic or therapeutic use.

Background

Multi-rAb™ Recombinant Secondary Antibodies are mixtures of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on the same IgG. Each recombinant clone in the final multiclonal mixture is carefully selected after rigorous characterization and screening to ensure the highest level of performance. ​The selected clones are mixed in various combinations, each of which is then functionally characterized in multiple applications to select the final multiclonal mixture with optimal performance. Each lot of Multi-rAb™ Secondary Antibodies is produced by mixing the same high performing recombinant clones every time to ensure that they have high batch-to-batch consistency, which in turn results in high reproducibility of experimental data.

Publications

Title
Adv Sci (Weinh); Lysosome-Featured Cell Aggregate-Released Extracellular Vesicles Regulate Iron Homeostasis and Alleviate Post-Irradiation Endothelial Ferroptosis for Mandibular Regeneration; Authors - Yuan-Yuan Li
Biochem Pharmacol; Discovery of a novel microtubule destabilizing agent targeting the colchicine site based on molecular docking; Authors - Jiangying Cai
bioRxiv; Comparative analysis of the syncytiotrophoblast in placenta tissue and trophoblast organoids using snRNA sequencing; Authors - Madeline M Keenen
Biochem Biophys Res Commun; Topical application of phenformin ameliorates the psoriasis-like inflammatory response via the inhibition of c-Myc expression in keratinocytes; Authors - Xue Leng
Adv Sci (Weinh); Spatial Transcriptomic Study Reveals Heterogeneous Metabolic Adaptation and a Role of Pericentral PPARα/CAR/Ces2a Axis During Fasting in Mouse Liver; Authors - Shiguan Wang
Adv Sci (Weinh); DAPK3 is Essential for DBP-Induced Autophagy of Mouse Leydig Cells; Authors - Si Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vanessa (Verified Customer) (11-24-2025)

We used this anti-mouse secondary antibody for immunohistochemistry in 50 and 100 µm frozen mouse brain sections, and it performed extremely well under both conditions. The antibody produced bright, clean fluorescence with excellent specificity and no noticeable background, even in thicker slices where nonspecific signal can be more common.

• Applications: Immunohistochemistry, Immunofluorescence
• Cell Tissue Type: Brain

FH

Igor (Verified Customer) (08-01-2025)

The background noise is low, and although it was not a strong signal, it was fine for my experiments with endothelial cells.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Endothelial cells