Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)

Multi-rAb Goat Anti-Mouse Recombinant Secondary Antibody (H+L) is a mixture of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on the mouse IgG heavy and light chains.

Applications

ELISA, WB, Dot blot

Conjugate

HRP

Host

Goat

Reactivity

Mouse

Publications

2

Cat no : RGAM001

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Validation Data Gallery

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  Various lysates were subjected to SDS-PAGE followed by western blot with U2AF2 mouse monoclonal antibody (68166-1-Ig) at 1:20000. Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM001 were used at 1:20000 for detection.

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  Various lysates were subjected to SDS-PAGE followed by western blot with CACYBP mouse monoclonal antibody (68161-1-Ig) at 1:20000. Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM001 were used at 1:20000 for detection.

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  Various lysates were subjected to SDS-PAGE followed by western blot with Catalase mouse monoclonal antibody (66765-1-Ig) at 1:10000. Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM001 were used at 1:20000 for detection.

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  Various lysates were subjected to SDS-PAGE followed by western blot with EIF3E mouse monoclonal antibody (67095-1-Ig) at dilution of 1:50000. Three batches of Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM001) were used at 1:20000 for detection.

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  Various lysates were subjected to SDS-PAGE followed by western blot with EFTUD2 mouse monoclonal antibody (67855-1-Ig, isotype IgG1) at dilution of 1:10000. RGAM001 (left) and competitor’s non cross-adsorbed HRP-Goat anti-mouse (H+L) secondary antibody (right) were both used at 0.05μg/mL for detection.

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  Various lysates were subjected to SDS-PAGE followed by western blot with Flag tag mouse monoclonal antibody (sigma M2, isotype IgG1) at dilution of 1:50000. RGAM001 (left) and competitor’s non cross-adsorbed HRP-Goat anti-mouse (H+L) secondary antibody (right) were both used at 0.05μg/mL for detection.

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  Various lysates were subjected to SDS-PAGE followed by western blot with Beta actin mouse monoclonal antibody (60008-1-Ig, isotype IgM) at dilution of 1:50000. RGAM001 (left) and competitor’s non cross-adsorbed HRP-Goat anti-mouse (H+L) secondary antibody (right) were both used at 0.05μg/mL for detection.

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  Various lysates were subjected to SDS-PAGE followed by western blot with Gelsolin mouse monoclonal antibody 66280-1-Ig (isotype IgA) at dilution of 1:5000. RGAM001 (left) and competitor’s non cross-adsorbed HRP-Goat anti-mouse (H+L) secondary antibody (right) were used at 1:20000 for detection.

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  Mouse total IgG, Mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgA monoclonal antibodies, Rat total IgG, Pig total IgG, Human total IgG, Bovine total IgG were coated at 100 ng/well. 0.125 μg/mL Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM001) was used for detection. The result indicates that RGAM001 strongly binds to all Mouse IgGs, Mouse IgM and IgA as well as Rat IgG. It shows weak reactivity for pig IgG and does not react with other species tested in the experiment. 

Product Information

RGAM001 targets Mouse IgG (H+L) in ELISA and Western blot. It can bind to all mouse IgG isotypes, mouse IgM and IgA as well as rat IgG. It shows minimal reactivity for rabbit, human, pig and bovine IgGs.

• Concentration: 1.0 mg/mL
• Applications: ELISA, WB, Dot blot
• Host Species: Goat
• Species Reactivity: Mouse
• Physical State: Liquid
• Suggested Dilution Range: 1:10,000-1:100,000 for ELISA; 1:10,000-1:20,0000 for western blotting with ECL substrates (1:10,000-1:40,000 is suggested for most system).
• Purity: The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads.
• Storage Buffer: PBS with 50% glycerol, 10 mg/mL BSA, 0.1% Proclin-300, pH 7.4.
• Storage Instructions: Store at -20°C. Stable for one year after shipment.
• Clonality: Multiclonal recombinant
• RRID: AB_3068333
• Note: This product is for research use only,not for diagnostic or therapeutic use.

Background

Multi-rAb Recombinant Secondary Antibodies are mixtures of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on the same IgG. Each recombinant clone in the final multiclonal mixture is carefully selected after rigorous characterization and screening to ensure the highest level of performance. ​The selected clones are mixed in various combinations, each of which is then functionally characterized in multiple applications to select the final multiclonal mixture with optimal performance. Each lot of Multi-rAb Secondary Antibodies is produced by mixing the same high performing recombinant clones every time to ensure that they have high batch-to-batch consistency, which in turn results in high reproducibility of experimental data.

Publications

Title
J Periodontol; Leukemia inhibitory factor protects against experimental periodontitis through immuno-modulations of both macrophages and periodontal ligament fibroblasts; Authors - Yanjing Ou
Mol Med; Targeting the NAT10/NPM1 axis abrogates PD-L1 expression and improves the response to immune checkpoint blockade therapy; Authors - Ge Qin

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