Tested Applications
| Positive WB detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24147-1-AP targets Multi tags in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21457 Product name: Recombinant Multi tags protein Source: e coli.-derived, PGEX-4T Tag: GST Sequence: EQKLISEEDLGEQKLISEEDLGEQKLISEEDLGHHHHHHGDYKDDDDKGDYKDDDDKGDYKDDDDKGHHHHHHGYPYDVPDYAGYPYDVPDYAGYPYDVPDYAGHHHHHHGFGKPIPNPLLGLDST Predict reactive species |
| Full Name | Multi tags |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2879441 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
This antibody recognize multi tags include: GST tag, 6*HIS tag, DDDDK tag, MYC tag and V5 tag.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Multi tags antibody 24147-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Multi Tags Polyclonal Antibody (Cat# 24147-1-AP), which recognizes GST, 6×His, DDDDK, MYC, and V5 tags, has 3 citations. These appear in Autophagy (viral replication/autophagy), Phytomedicine (inflammasome/chronic asthma), and Oxidative Medicine and Cellular Longevity (ferroptosis/intestinal damage). The cited applications are primarily Western blot, spanning research fields of virology, immunology, and oxidative stress biology.
| Species | Application | Title |
|---|---|---|
Autophagy Cytosolic DNA-induced CGAS-STING1-dependent autophagy promotes SFTSV and EV-71 replication. | ||
Phytomedicine Icariside II targets M2 macrophages by regulating the inflammasome and profibrotic signaling in chronic asthma. | ||
Oxid Med Cell Longev Pterostilbene Confers Protection against Diquat-Induced Intestinal Damage with Potential Regulation of Redox Status and Ferroptosis in Broiler Chickens |
Reviews
What customers say
One customer rated this antibody 3 out of 5 stars. They used it on a Western blot containing C-terminal His6 and C-terminal FLAG (DDDDK) epitope tags, but only the FLAG-tagged proteins were detected; the His6-tagged proteins were not visible. The antibody was used at a 1:5000 dilution. Overall, the review highlights a limitation in detecting His-tagged proteins in this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ryan (Verified Customer) (03-11-2022) | I tried the Multi tags Rabbit PolyAB on a western blot that had C-terminal His6 and C-terminal FLAG (DDDDK) epitope tags. Only FLAG tagged proteins were visible.
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