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MSI2 Polyclonal antibody

MSI2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10770-1-AP

Publications (14)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, RIP, ELISA

Synonyms

Musashi-2, MSI2H, Musashi 2, RNA-binding protein Musashi homolog 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHC

WB analysis of PC-12 using 10770-1-AP
PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of COLO 320 using 10770-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of C6 using 10770-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of C6 using 10770-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of PC-12 using 10770-1-AP
PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 10770-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of PC-12 using 10770-1-AP
PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 10770-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 10770-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of MCF-7 using 10770-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody) at dilution of 1:500.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary tumor using 10770-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary tumor using 10770-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse liver using 10770-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse liver using 10770-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 10770-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 10770-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (14)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	PC-12 cells, C6 cells, COLO 320 cells, MCF-7 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human ovary tumor tissue, human colon cancer tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10770-1-AP targets MSI2 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1213 Product name: Recombinant human Musashi-2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC001526 Sequence: MEANGSQGTSGSANDSQHDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADPASVDKVLGQPHHELDSKTIDPKVAFPRRAQPKMVTRTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVMFPPGTRGRARGLPYTMDAFMLGMGMLGYPNFVATYGRGYPGFAPSYGYQFPGFPAAAYGPVAAAAVAAARGSGSNPARPGGFPGANSPGPVADLYGPASQDSGVGNYISAASPQPGSGFGHGIAGPLIATAFTNGYH Predict reactive species
Full Name	musashi homolog 2 (Drosophila)
Calculated Molecular Weight	35 kDa
Observed Molecular Weight	37-40 kDa
GenBank Accession Number	BC001526
Gene Symbol	MSI2
Gene ID (NCBI)	124540
RRID	AB_2147935
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96DH6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MSI2 is an RNA-binding protein that regulates the expression of target mRNAs at the translation level. It binds to RNA via 2 ribonucleoprotein-type RNA recognition motifs in their N-termini, to consensus motifs in mRNAs to inhibit transcription. It may play a role in the proliferation and maintenance of stem cells in the central nervous system. MSI2 can regulate normal hematopoiesis and promote aggressive myeloid leukemia

Protocols

Product Specific Protocols
IHC protocol for MSI2 antibody 10770-1-AP	Download protocol
IP protocol for MSI2 antibody 10770-1-AP	Download protocol
WB protocol for MSI2 antibody 10770-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

MSI2 Polyclonal antibody (Cat# 10770-1-AP) has 14 total citations published in journals including Science, Nature Communications, Oncogene, EMBO Journal, Journal of Biological Chemistry, and International Journal of Biological Sciences. Associated research fields encompass cancer biology (esophageal, pancreatic, breast, colon, and liver cancers), Zika viral replication, diabetes, glioma, RNA-binding protein function, Wnt/Hedgehog signaling, circadian rhythms, and blood-tumor barrier biology.

Species	Application	Title
human	WB,IF	Science Neurodevelopmental protein Musashi-1 interacts with the Zika genome and promotes viral replication. Authors - Pavithra L Chavali View Article
mouse	WB	Nat Commun 5'tRNA-derived fragments modulate β-cell homeostasis and islet macrophage activation in type 2 diabetes. Authors - Cristina Cosentino View Article
mouse,human	WB,IP,IHC	MedComm (2020) CircMALAT1 promotes cancer stem-like properties and chemoresistance via regulating Musashi-2/c-Myc axis in esophageal squamous cell carcinoma Authors - Zitong Zhao View Article
human	WB,IP,IHC	Research (Wash D C) A Circular RNA Promotes Tumor Metastasis through Stabilizing MSI2 Protein in Pancreatic Ductal Adenocarcinoma. Authors - Zhang Li View Article
mouse,human	WB,RIP,IHC,IF	Int J Biol Sci Prolonged DEHP exposure enhances the stemness and metastatic potential of TNBC cells in an MSI2-dependent manner Authors - Mahendra Jadhao KD Validated View Article
human	WB	Oncogene β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells Authors - M Wagstaff KD Validated View Article

Reviews

5.0 out of 5

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1 verified customer reviews

What customers say

One reviewer (5/5) used this antibody at 1:1000 dilution for Western Blot on B cells. They noted a non-specific band above the target, but it was far enough away not to interfere with interpretation. The two specific bands decreased as expected with treatment, validating their omics results and confirming the bands of interest. Overall, the antibody performed well for their application.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Annabel (Verified Customer) (12-23-2025)

Produces a non-specific band above, but this is far enough away not to affect the image. The two bands below both go down as expected with treatment, validating our omics results, so confident these are the bands of interest.