ChromoTek Myc-Trap® Agarose

Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.

Specificity

Myc-Tag

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Agarose

Cat no : yta

Synonyms

Myc tag, Myc, Myc-trap, EQKLISEEDL



Product Information

Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.

DescriptionImmunoprecipitation of Myc-tagged proteins and their interacting factors with anti-Myc Nanobody conjugated to beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Elution with peptide

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetMyc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound.
Binding capacity17.5 μg of recombinant Myc-tagged protein (~42 kDa) per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution buffer2x Myc peptide.
SDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) 1x Myc-tag: Dissociation constant KD of 500 nM
2x Myc-tag: Dissociation constant KD of 0.5 nM
Compatibility with mass spectrometryThe Myc-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631369
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Publications

ApplicationTitle
IP

Cell

Regulatory control of the resolution of DNA recombination intermediates during meiosis and mitosis.

Authors - Joao Matos
IP

Nat Genet

MTF2 recruits Polycomb Repressive Complex 2 by helical-shape-selective DNA binding.

Authors - Matteo Perino
IP

J Hepatol

Immunity-related GTPase induces lipophagy to prevent excess hepatic lipid accumulation.

Authors - Kristin Schwerbel
IP

Nat Neurosci

TREM2 interacts with TDP-43 and mediates microglial neuroprotection against TDP-43-related neurodegeneration.

Authors - Manling Xie
IP

Nat Cell Biol

FERARI is required for Rab11-dependent endocytic recycling.

Authors - Jachen A Solinger
IP

Nat Cell Biol

Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.

Authors - Boris Simonetti

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Frédéric (Verified Customer) (05-05-2022)

I'm very satisfied with the Myc-Trap agarose. The result is excellent and it is easy to use.

  • Applications: Immunoprecipitation
  • Cell Tissue Type: human erythroleukemia cell line (HEL)