Myh9 Polyclonal antibody

• Featured Product
• KD/KO Validated

Myh9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 14844-1-AP

Synonyms

Myosin heavy chain, non-muscle IIa, Myhn1, Myhn 1, D0Jmb2, Cellular myosin heavy chain, type A

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Validation Data Gallery

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  WB analysis using 14844-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14844-1-AP (Myh9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 14844-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14844-1-AP (Myh9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14844-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14844-1-AP (Myh9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MDCK using 14844-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 14844-1-AP (Myh9 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, SGC-7901 cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

14844-1-AP targets Myh9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, canine samples.

• Tested Reactivity: human, mouse, canine
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, heavy polypeptide 9, non-muscle
• Calculated Molecular Weight: 226 kDa
• Observed Molecular Weight: 224 kDa
• GenBank Accession Number: NM_022410
• Gene Symbol: Myh9
• Gene ID (NCBI): 17886
• RRID: AB_2147311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYH9, also named as NMMHC-A and NMMHC-IIA, is a cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. It plays an important role in cytoskeleton reorganization, focal contacts formation (in the margins but not the central part of spreading cells), and lamellipodial retraction, during cell spreading.

Publications

Species	Application	Title
human, mouse	WB, IF	Nat Commun; The non-muscle-myosin-II heavy chain Myh9 mediates colitis-induced epithelium injury by restricting Lgr5+ stem cells.; Authors - Bing Zhao
mouse	IF, WB	Theranostics; Trio cooperates with Myh9 to regulate neural crest-derived craniofacial development.; Authors - Shuyu Guo ; KD Validated
human	WB	Cell Cycle; PMID: 23974104; Authors - Tyler J W Robinson
mouse	WB,IHC	Neuromolecular Med; Generation and characterization of mice with Myh9 deficiency.; Authors - Mhatre Anand N AN ; KO Validated
human	IHC	Clin Genet; Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population.; Authors - Zhongxian Zhu
rat	WB	Biomed Pharmacother; Tandem mass tag (TMT) proteomic analysis of fetal lungs revealed differential expression of tight junction proteins in a rat model of congenital diaphragmatic hernia.; Authors - Xue Li